CAJ | 학술논문

目的：建立测定Beagle犬血浆中灯盏花素浓度的反相高效液相色谱法和液相-质谱联用法，并将其进行多参数的两两比较。方法：采用液-液萃取法处理血清，反相高效液相色谱法测定，流动相为甲醇-水（pH3.0）=42∶58， Eclipse XDB-C18为固定相；将同样的样品用液相-质谱法开展平行检测；将所测得的数据做两两对比，采用SPSS统计软件进行分析。结果：2种方法测定的数据具有良好的相关性（r=0.982，P>0.05）；最低检测限前者为0.05μg/mL，后者为0.01μg/mL，反相高效液相色谱法的最低检测限和灵敏度不及液相-质谱联用法。结论：反相高效液相色谱法可用于灯盏花素毒代动力学研究，与液相-质谱联用法检测结果无显著差异，且费用较低，不失为一种低成本且行之有效的检测方法。
목적：건립측정Beagle견혈장중등잔화소농도적반상고효액상색보법화액상-질보련용법，병장기진행다삼수적량량비교。방법：채용액-액췌취법처리혈청，반상고효액상색보법측정，류동상위갑순-수（pH3.0）=42∶58， Eclipse XDB-C18위고정상；장동양적양품용액상-질보법개전평행검측；장소측득적수거주량량대비，채용SPSS통계연건진행분석。결과：2충방법측정적수거구유량호적상관성（r=0.982，P>0.05）；최저검측한전자위0.05μg/mL，후자위0.01μg/mL，반상고효액상색보법적최저검측한화령민도불급액상-질보련용법。결론：반상고효액상색보법가용우등잔화소독대동역학연구，여액상-질보련용법검측결과무현저차이，차비용교저，불실위일충저성본차행지유효적검측방법。
Objective: To establish RP-HPLC and LC-MS/MS methods for determination of blood concentration of scutellarin in Beagle dogs, and compare them by methodology parameters and several sample test results. Meth-ods: LC/LC extract was used to prepare samples and RP-HPLC was used to determine scutellarin. Mobile phase was methanol/water(pH3.0)=42∶58, and the column was Eclipse XDB-C18. The result was compared with that of the same samples determined by LC-MS/MS method. The data were analysed by SPSS program. Results: A satis-fied correlation was obtained from the data of the two methods(r=0.982, P>0.05). The lower limit of quantification of RP-HPLC and LC-MS/MS methods were 0.05 and 0.01 μg/mL, respectively. So the sensitivity of LC-MS/MS method is better. Conclusion: This method was suitable for the determination of blood concentration in Beagle dogs for toxicokinetics of scutellarin. There was no significant difference between the data of the two methods, and the cost of the HPLC method was lower relatively, and the HPLC method was practicable though not best.