CAJ | 학술논문

目的：观察雷公藤甲素对胰腺癌细胞BxPC-3和PANC-1生长抑制及诱导细胞凋亡作用，探讨雷公藤甲素抗胰腺癌的机制。方法：雷公藤甲素处理BxPC-3和PANC-1细胞后，用MTT法检测细胞的生长抑制，用流式细胞术检测细胞的凋亡率，用罗丹明123和DCFH-DA染色方法测定细胞线粒体膜跨膜电位变化和活性氧（ROS）的产生，用Western印迹检测Bcl-2、Bax蛋白表达的变化。结果：雷公藤甲素对胰腺癌细胞BxPC-3和PANC-1具有生长抑制和诱导细胞凋亡的作用，且呈时间和剂量依赖关系；处理72 h后，胰腺癌细胞线粒体跨膜电位明显下降，Bax表达上调， Bcl-2表达下降。结论：雷公藤甲素能有效抑制胰腺癌细胞增殖，通过增强线粒体通透性诱导细胞凋亡。
목적：관찰뢰공등갑소대이선암세포BxPC-3화PANC-1생장억제급유도세포조망작용，탐토뢰공등갑소항이선암적궤제。방법：뢰공등갑소처리BxPC-3화PANC-1세포후，용MTT법검측세포적생장억제，용류식세포술검측세포적조망솔，용라단명123화DCFH-DA염색방법측정세포선립체막과막전위변화화활성양（ROS）적산생，용Western인적검측Bcl-2、Bax단백표체적변화。결과：뢰공등갑소대이선암세포BxPC-3화PANC-1구유생장억제화유도세포조망적작용，차정시간화제량의뢰관계；처리72 h후，이선암세포선립체과막전위명현하강，Bax표체상조， Bcl-2표체하강。결론：뢰공등갑소능유효억제이선암세포증식，통과증강선립체통투성유도세포조망。
Objective: To determine whether triptolide inhibits proliferation and induces apoptosis of pancreatic cancer cells. Methods: The effects of triptolide on the antiproliferative activity of pancreatic cancer cells BxPC-3 and PANC-1 were determined by MTT assays. Apoptosis of pancreatic cancer cells induced by triptolide were de-termined by flow cytometry analysis. Mitochondrial membranere potential and ROS production analysis were detect-ed by Rhodamine 123 and DCFH-DA. The effects of triptolide on expression levels of Bcl-2 and Bax were deter-mined by Western blotting. Results: Triptolide displayed inhibition activity of cells growth and induction of apopto-sis on pancreatic cancer cells. Triptolide induced the loss of mitochondrial membrane potential by regulating the expression levels of Bcl-2 family proteins. Conclusion: The results suggest that triptolide may inhibit proliferation and induce apoptosis of pancreatic cancer cells by inducing the loss of mitochondrial membrane potential.