CAJ | 학술논문

目的：研究尾悬吊模拟失重环境对大鼠血清胃泌素（GAS）、胃动素（MTL）和胃窦Cajal间质细胞（ICC）的影响。方法：健康雄性Wistar大鼠64只随机分为8组（n=8），按模拟失重时相分别设为6 h、12 h、1 d、2 d、3 d、5 d、7 d和0 h（地面对照组）组，采用尾悬吊法建立模拟失重动物模型。应用放免法测定血清GAS、MTL浓度，免疫组化和RT-PCR技术检测各组大鼠胃窦组织中c-kit蛋白和mRNA表达情况。结果：尾悬吊6、12 h阶段，血清GAS浓度明显升高，与对照组比较差异有统计学意义（P<0.05）；随尾悬吊时相延长，血清GAS含量呈逐渐下降趋势，与正常对照组水平相近。尾悬吊各组大鼠血清MTL浓度均升高，12 h以后各组MTL浓度值与对照组比较，差异有统计学意义（P<0.05）。免疫组化结果显示，c-kit蛋白阳性表达为棕褐色，主要分布在ICC胞体和突起，正常对照组大鼠胃窦肌层ICC （ICC-MY）呈连续性浓染，肌层内ICC（ICC-IM）亦明晰可见；6 h~5 d的尾悬吊过程中，大鼠胃窦ICC-MY连续性出现中断现象且逐渐明显，染色逐渐减弱，ICC-IM染色减弱的同时c-kit阳性ICC也明显减少；7 d组胃窦组织中c-kit蛋白表达有所恢复。模拟失重各组及对照组大鼠胃窦组织中均有c-kit mRNA表达，尾悬吊6、12 h组的c-kit mRNA表达明显下降，与对照组比较，差异有统计学意义（P<0.05），1~3 d组逐渐恢复，5 d组又出现明显下降（P<0.05），7 d组c-kit mRNA表达值明显复升。结论：尾悬吊模拟失重对大鼠血清GAS、MTL和胃窦Cajal间质细胞c-kit蛋白及mRNA表达造成明显影响，可能导致胃动力障碍。
목적：연구미현조모의실중배경대대서혈청위비소（GAS）、위동소（MTL）화위두Cajal간질세포（ICC）적영향。방법：건강웅성Wistar대서64지수궤분위8조（n=8），안모의실중시상분별설위6 h、12 h、1 d、2 d、3 d、5 d、7 d화0 h（지면대조조）조，채용미현조법건립모의실중동물모형。응용방면법측정혈청GAS、MTL농도，면역조화화RT-PCR기술검측각조대서위두조직중c-kit단백화mRNA표체정황。결과：미현조6、12 h계단，혈청GAS농도명현승고，여대조조비교차이유통계학의의（P<0.05）；수미현조시상연장，혈청GAS함량정축점하강추세，여정상대조조수평상근。미현조각조대서혈청MTL농도균승고，12 h이후각조MTL농도치여대조조비교，차이유통계학의의（P<0.05）。면역조화결과현시，c-kit단백양성표체위종갈색，주요분포재ICC포체화돌기，정상대조조대서위두기층ICC （ICC-MY）정련속성농염，기층내ICC（ICC-IM）역명석가견；6 h~5 d적미현조과정중，대서위두ICC-MY련속성출현중단현상차축점명현，염색축점감약，ICC-IM염색감약적동시c-kit양성ICC야명현감소；7 d조위두조직중c-kit단백표체유소회복。모의실중각조급대조조대서위두조직중균유c-kit mRNA표체，미현조6、12 h조적c-kit mRNA표체명현하강，여대조조비교，차이유통계학의의（P<0.05），1~3 d조축점회복，5 d조우출현명현하강（P<0.05），7 d조c-kit mRNA표체치명현복승。결론：미현조모의실중대대서혈청GAS、MTL화위두Cajal간질세포c-kit단백급mRNA표체조성명현영향，가능도치위동력장애。
Objective: To investigate the effects of simulated weightlessness on serum gastrin, motilin and inter-stitial cells of Cajal(ICC) in rat gastric antrum. Methods: Sixty four adult male Wistar rats were randomly divid-ed into 8 experimental groups(n=8) including suspension for 6 h, 12 h, 1 d, 2 d, 3 d, 5 d, 7 d and 0 h(con-trol). Tail-suspension was used to simulate the weightlessness animal model. Serum concentrations of gastrin and motilin were determined by radioimmunoassay. The c-kit protein and mRNA in rat gastric antrum were detected by immunohistochemical assay and RT-PCR technique. Results: Compared with the control group, the level of se-rum gastrin increased significantly in the early phase of tail-suspension with a peak value at 6 and 12 h(P<0.05) followed by declination and trend to normal range during the sustained suspension, and the level of serum motilin obviously increased in each groups except 6 h group(P<0.05). The immunohistochemistry assay showed that c-kit protein was stained as brown particles and mainly located in cell soma and dentrites of ICC. The myen-teric plexus ICC(ICC-MY) and intra-muscular ICC(ICC-IM) were deeply stained normally in rat gastric antrum. The expression of c-kit protein was obviously weakened during the period of 6 h to 5 d tail suspension, and the continuity of ICC-MY was gradually interrupted. However, the expression of c-kit protein was up-regulated in 7 d group. The semi-quantitative RT-PCR showed that the c-kit mRNA in rat gastric antrum was significantly down-regulated at 6 and 12 h of suspension compared with the control group(P<0.05), followed by a gradually re-covery at 1~3 d of suspension, then the c-kit mRNA was again significantly down-regulated at 5 d of suspension (P<0.05). Interestingly, the expression of c-kit mRNA was markedly increased at 7 d of tai-suspension. Conclu-sion: This study showed that the simulated weightlessness by tail-suspension could disturb the serum gastrin and motilin levels and affect the c-kit expression both at protein and mRNA levels in rat gastric antrum, and thus the gastric motility may be impaired.