CAJ | 학술논문

目的：分离筛选能够产生抑菌活性物质的海洋放线菌，并进行生理生化和16S rDNA鉴定。方法：用分离培养基培养海洋放线菌，并筛选出能够产生抑菌活性物质的菌株，对所筛选菌株的形态特征、生理生化特性进行鉴定分析；采用通用引物27F、1492R扩增该菌株的16S rDNA，对测序结果进行分析；采用Neighbor-Joining（N-J）法构建系统发育进化树。结果：筛选到一株对金黄色葡萄球菌、大肠杆菌、白色念珠菌具有较强抗性的海洋放线菌F1，该菌株好氧，中度嗜盐，在高氏Ⅰ号培养基上呈白色绒粉状，16S rDNA序列比对表明该菌株与田无链霉菌（Streptomyces tanashiensis）NR043369的相似度为99%。结论：筛选到的菌株F1是一株海洋来源的放线菌，与田无链霉菌NR043369的同源性较高，可能属海洋链霉菌属，对金黄色葡萄球菌等病原菌具有较强的抑菌活性。
목적：분리사선능구산생억균활성물질적해양방선균，병진행생리생화화16S rDNA감정。방법：용분리배양기배양해양방선균，병사선출능구산생억균활성물질적균주，대소사선균주적형태특정、생리생화특성진행감정분석；채용통용인물27F、1492R확증해균주적16S rDNA，대측서결과진행분석；채용Neighbor-Joining（N-J）법구건계통발육진화수。결과：사선도일주대금황색포도구균、대장간균、백색념주균구유교강항성적해양방선균F1，해균주호양，중도기염，재고씨Ⅰ호배양기상정백색융분상，16S rDNA서렬비대표명해균주여전무련매균（Streptomyces tanashiensis）NR043369적상사도위99%。결론：사선도적균주F1시일주해양래원적방선균，여전무련매균NR043369적동원성교고，가능속해양련매균속，대금황색포도구균등병원균구유교강적억균활성。
Objective: To isolate and screen antibacterial actinomycetes from the ocean, and make a preliminary study on its morphological, physiological and biochemical properties and 16S rDNA identification. Methods: Anti-bacterial actinomycetes from the ocean were isolated by the use of selective medium and the morphological physio-logical and biochemical properties were identified and analyzed. 16S rDNA sequence for actinomycetes strains was amplified by prime pairs 27F and 1492R, the acquired data were used to analyze sequence, and using Neigh-bor-Joining method to construct phylogenic tree. Results: An actinomycete strain named F1 was found to have re-markable activities to Staphylococcus aureus, Escherichia coli and Candidia albicans. The strain of F1 was aerobic and moderately halophilic, white velvet powder on the Gause's medium. The sequence analysis of 16S rDNA showed that F1 was closely to Streptomyces tanashiensis NR043369, with the similarity of 99%. Conclusion: The marine actinomycete strain F1 has a relatively high homology compared with S.tanashiensis NR043369. The strain F1 can produce some active substances inhibiting pathogenic bacteria including S.aureus, E.coli.