CAJ | 학술논문

目的：利用临床耐药粪肠球菌分离裂解性噬菌体，为应用噬菌体治疗耐药粪肠球菌感染提供基础。方法：利用噬菌斑实验分离噬菌体并观察噬菌斑形态；双层平板培养法测定噬菌体效价、最佳感染复数及一步生长曲线；负染法电镜观察噬菌体形态；蛋白酶K/SDS法提取噬菌体基因组，酶切处理后琼脂糖凝胶电泳分析。结果：分离出一株噬菌体IME-EF1，该噬菌体能裂解多株临床分离的粪肠球菌；电镜观察呈蝌蚪形，最佳感染复数为1；通过绘制一步生长曲线，证明该噬菌体感染后的潜伏期为25 min，爆发期为35 min，裂解量为60 pfu。结论：研究结果表明利用临床分离的耐药粪肠球菌分离裂解性噬菌体是可行的，有望为耐药粪肠球菌的抗生素替代疗法奠定基础。
목적：이용림상내약분장구균분리렬해성서균체，위응용서균체치료내약분장구균감염제공기출。방법：이용서균반실험분리서균체병관찰서균반형태；쌍층평판배양법측정서균체효개、최가감염복수급일보생장곡선；부염법전경관찰서균체형태；단백매K/SDS법제취서균체기인조，매절처리후경지당응효전영분석。결과：분리출일주서균체IME-EF1，해서균체능렬해다주림상분리적분장구균；전경관찰정과두형，최가감염복수위1；통과회제일보생장곡선，증명해서균체감염후적잠복기위25 min，폭발기위35 min，렬해량위60 pfu。결론：연구결과표명이용림상분리적내약분장구균분리렬해성서균체시가행적，유망위내약분장구균적항생소체대요법전정기출。
Objective: To isolate bacteriocidal phage against multi-antibiotics resistant Enterococcus faecalis. Meth-ods: A strain of E. faecalis which was clinically separated and proved to be multi-antibiotics resistant was used as host to isolate bacteriophage from hospital sewage. The isolated phage was characterized by observing the shape of plaque, titering on double-layer agar culture plate, the optimal multiplicity of infection, one-step growth kinet-ics, and electron microscopic observation. Results: The isolated bacteriophage from hospital sewage was lytic to many strains of E. faecalis and E. facium. The electron microscopic observation showed that the bacteriophage shaped like a tadpole. One-step growth kinetics indicated that the latent infection time was 25 minutes and burst-ed at 35 minutes. Conclusion: The results of this research demonstrated that isolating bacteriophage using clinical strains of E. faecalis was possible and it can be anticipated that the bacteriophage could be adopted to treat anti-biotics-resistant E. faecalis infection.