CAJ | 학술논문

目的：比较分析大豆素与葛根素体外抗氧化及抑制NO、NOS活性机制。方法：用1滋g·mL-1 LPS诱导体外培养的RAW264.7细胞，制备呼吸爆发、自由基反应模型。采用MTT法检测大豆素及葛根素的细胞毒性；采用DCFH-DA探针及激光共聚焦显微镜观察被试药的抗氧化能力；采用Griess法检测细胞培养体系中NO水平；采用化学比色法测定细胞内T-NOS含量。结果：大豆素与葛根素均能显著抑制LPS诱导RAW264.7细胞NO释放及T-NOS表达。结论：大豆素与葛根素的抗氧自由基活性接近，有望作为葛根素的替代品使用。
목적：비교분석대두소여갈근소체외항양화급억제NO、NOS활성궤제。방법：용1자g·mL-1 LPS유도체외배양적RAW264.7세포，제비호흡폭발、자유기반응모형。채용MTT법검측대두소급갈근소적세포독성；채용DCFH-DA탐침급격광공취초현미경관찰피시약적항양화능력；채용Griess법검측세포배양체계중NO수평；채용화학비색법측정세포내T-NOS함량。결과：대두소여갈근소균능현저억제LPS유도RAW264.7세포NO석방급T-NOS표체。결론：대두소여갈근소적항양자유기활성접근，유망작위갈근소적체대품사용。
This study was aimed to compare the activation of daidzein and puerarin on antioxidation, NO and NOS suppression in vitro . The RAW264 . 7 cell line was used to prepare radical reaction model induced by LPS (1 μg/mL). MTT method was adopted to detect cytotoxicity of daidzein and puerarin. The DCFH-DA probe and confocal microscopy were used to examine the antioxidant ability of daidzein and puerarin. Griess reagent was adopted to test the NO level in the culture medium. And chemical colorimetry was used to detect the content in RAW264.6 cells. The results showed that daidzein and puerarin can significantly suppress the NO and T-NOS expression in RAW264.7 cells induced by LPS. It was concluded that there was no difference on the activation of antioxidant free radical between daidzein and puerarin . In this regard , daidzein can be the used as substitutes of puerarin .