CAJ | 학술논문

在pH＝7．00的B-R缓冲溶液中，人血清白蛋白（HSA）均能与6-苄基腺嘌呤（6-BA）和激动素（K T ）这两种天然细胞分裂素作用并产生不同强度的荧光猝灭信号．首先通过计算荧光偏振信号获得其荧光偏振度（p）和荧光各向异性（r）来区分6-苄基腺嘌呤和激动素这两种天然细胞分裂素，同时利用荧光猝灭信号和双标准曲线计量分析法对2种天然细胞分裂素进行同时测定，并将该方法应用于市售苦瓜和番茄样品表皮中6-苄基腺嘌呤和激动素的同时测定，结果令人满意．
재pH＝7．00적B-R완충용액중，인혈청백단백（HSA）균능여6-변기선표령（6-BA）화격동소（K T ）저량충천연세포분렬소작용병산생불동강도적형광졸멸신호．수선통과계산형광편진신호획득기형광편진도（p）화형광각향이성（r）래구분6-변기선표령화격동소저량충천연세포분렬소，동시이용형광졸멸신호화쌍표준곡선계량분석법대2충천연세포분렬소진행동시측정，병장해방법응용우시수고과화번가양품표피중6-변기선표령화격동소적동시측정，결과령인만의．
pH=7 .00 B-R buffer solution ,HSA could react with natural cytokinins of 6-benzylaminourine and kinetin and produced different fluorescence quenching spectra signals .The degree of fluorescence polarization (p) and the degree of fluorescence anisotropy (r) were calculated based on the measure-ments of the polarized fuorescence signals to distinguish two natural cytokinins of 6-benzylaminourine and kinetin .Simultaneously based on the fuorescence quenching spectra signals and the double standard curve method ,a novel method for simultaneous determination of 6-benzylaminourine and kinetin was de-veloped .The method was applied to simultaneous determination of 6-benzylaminourine and kinetin in balsam pear skin and tomato skin with satisfactory results .