CAJ | 학술논문

为了解浙江省规模化猪场患病猪副猪嗜血杆菌的耐药性，采用K-B琼脂法对73株HPS浙江株进行了18种临床药物的敏感性试验，同时应用PCR技术对11个相关耐药基因进行扩增、测序和比对分析。药敏试验结果显示：对磺胺类耐药的分离株比例高达86．30％，对四环素类、氨基糖苷类、喹诺酮类、β-内酰胺类和氯霉素类耐药的比例分别为65．75％，60．27％，50．68％，50．68％，46．58％，对大环内酯类耐药仅为28．77％。 PCR分析显示：97．26％的菌株中检测到耐药基因，多重耐药的占84．93％。其中喹诺酮类耐药基因Aac（6′）-1b的检出率为76．71％，氨基糖苷类耐药基因AadA1的检出率为65．75％，磺胺类耐药基因Sul1和Sul2的检出率为45．2％和10．96％，氯霉素类耐药基因Flor、CmLA、Cat1的检出率为15．07％，8．22％，58．9％，四环素类耐药基因 TetA和 TetB 的检出率为4．11％和49.32％，β-内酰胺类耐药基因Tem-11的检出率为35．62％，大环内酯类耐药基因ErmB的检出率为1．37％。耐药基因型和表型符合率为：磺胺类62．07％，β-内酰胺类74．29％，氯霉素类81．48％，四环素类75.00％，大环内酯类0，喹诺酮类75．68％，氨基糖苷类72．09％。因此，HPS在浙江规模化猪场对临床药物已产生了耐药性，通过耐药基因检测结果确定耐药菌株具有一定的参考价值。
위료해절강성규모화저장환병저부저기혈간균적내약성，채용K-B경지법대73주HPS절강주진행료18충림상약물적민감성시험，동시응용PCR기술대11개상관내약기인진행확증、측서화비대분석。약민시험결과현시：대광알류내약적분리주비례고체86．30％，대사배소류、안기당감류、규낙동류、β-내선알류화록매소류내약적비례분별위65．75％，60．27％，50．68％，50．68％，46．58％，대대배내지류내약부위28．77％。 PCR분석현시：97．26％적균주중검측도내약기인，다중내약적점84．93％。기중규낙동류내약기인Aac（6′）-1b적검출솔위76．71％，안기당감류내약기인AadA1적검출솔위65．75％，광알류내약기인Sul1화Sul2적검출솔위45．2％화10．96％，록매소류내약기인Flor、CmLA、Cat1적검출솔위15．07％，8．22％，58．9％，사배소류내약기인 TetA화 TetB 적검출솔위4．11％화49.32％，β-내선알류내약기인Tem-11적검출솔위35．62％，대배내지류내약기인ErmB적검출솔위1．37％。내약기인형화표형부합솔위：광알류62．07％，β-내선알류74．29％，록매소류81．48％，사배소류75.00％，대배내지류0，규낙동류75．68％，안기당감류72．09％。인차，HPS재절강규모화저장대림상약물이산생료내약성，통과내약기인검측결과학정내약균주구유일정적삼고개치。
This study was conducted to investigate the resistance to common antibiotics and genes of Haemophi-lus parasuis isolated from the scaled pig farms in Zhejiang province .A total of 73 Zhejiang H.parasuis isolates were tested for their resistance to 18 antibiotics by K-B method.PCR was used to detect related genes .The amplified products were sequenced and aligned .The results showed that 86 .30% isolates were resistant to sulfonamides , 65.75%,60.27%,50.68%,50.68%and 46.58%isolates were resistant to tetracyclines ,aminoglycosides,quino-lones,β-lactam antibiotics,chloramphenicols,respectively,while only 28.77%isolates were resistant to macrolides. Resistance genes were detected in 97.26%isolates,and multiple resistance genes were found in 84.93%isolates. The rates of resistance genes Aac(6′)-1b,AadA1,Sul1,Sul2,Flor,CmLA,Cat1,TetA,TetB,Tem-11 and ErmB were 76.71%,65.75%,45.2%,10.96%,15.07%,8.22%,58.9%,4.11%,49.32%,35.62%and 1.37%,respec-tively.The coincidence of phenotype and genotype for the isolates to resist sulfonamides ,β-lactam antibiotics,chlor-amphenicols, tetracyclines, macrolides, quinolones and aminoglycoside antibiotics were 62.07%, 74.29%, 81.48%,75.00%,0,75.68%and 72.09%,respectively.Therefore,H.parasuis has developed resistance to clini-cally commonly used antibiotics and the results from analysis of drug-resistant gene could be used as reference to determine resistance bacteria .