CAJ | 학술논문

从剑麻叶片组织内分离获得6株内生细菌，其中JM-3菌株对离体苹果枝条上苹果腐烂病菌的扩展致病有较好的抑制效果。 JM-3菌株在培养过程中能产生抑菌物质，其无菌发酵滤液能抑制腐烂病菌菌丝的生长和分生孢子的萌发，导致菌丝和芽管膨大畸形，最终导致菌丝破裂、原生质外流。 JM-3菌株对多种果树和农作物病原真菌菌丝的生长都有明显的抑制作用。经16S rDNA序列分析和RiboPrinter分析，鉴定菌株JM-3为枯草芽孢杆菌。
종검마협편조직내분리획득6주내생세균，기중JM-3균주대리체평과지조상평과부란병균적확전치병유교호적억제효과。 JM-3균주재배양과정중능산생억균물질，기무균발효려액능억제부란병균균사적생장화분생포자적맹발，도치균사화아관팽대기형，최종도치균사파렬、원생질외류。 JM-3균주대다충과수화농작물병원진균균사적생장도유명현적억제작용。경16S rDNA서렬분석화RiboPrinter분석，감정균주JM-3위고초아포간균。
Six isolates of endophytic bacterial strains were isolated from healthy leaves of Agavesisalana Per-rine,in which the strain JM-3 inhibited canker lesion expansion of Valsa ceratosperma on excised twigs significantly . The cell-free fermentation liquid of JM-3 inhibited mycelium growth and conidial germination of Valsa ceratosperma. The isolates produced anti-fungi substances , which caused malformation of mycelia and germtubes .The malformed mycelium burst and protoplasm flow-out finally.JM-3 also showed clear inhibiting effect against mycelial growth of pathogen from several fruit trees and crop .JM-3 was identified as Bacillus subtilis based on 16S rDNA sequencing and RiboPrinter results .