CAJ | 학술논문

利用分子标记辅助选择技术改良武运粳7号的条纹叶枯病抗性，培育抗条纹叶枯病的粳稻新种质。以日本优质粳稻品种关东194为条纹叶枯病抗性基因Stv-bi的供体，采用定向回交育种策略，改良高产、感病品种武运粳7号的条纹叶枯病抗性。在连续回交和自交过程中，利用与Stv-bi紧密连锁的SCAR标记进行跟踪检测，并结合条纹叶枯病田间抗性鉴定以及农艺性状的系统选择，将抗性基因Stv-bi转移到武运粳7号中。采用分子标记辅助选择，从BC3 F3世代中筛选出14个具有Stv-bi纯合基因型的改良株系。其综合性状与武运粳7号十分相近，有的性状甚至优于武运粳7号。利用定向回交和分子标记辅助选择相结合的技术体系，可显著提高抗性改良的预见性和准确性，缩短育种年限、加快育种进程。新育成的武运粳7号抗性改良系，为条纹叶枯病抗性育种提供了重要的遗传资源。
이용분자표기보조선택기술개량무운갱7호적조문협고병항성，배육항조문협고병적갱도신충질。이일본우질갱도품충관동194위조문협고병항성기인Stv-bi적공체，채용정향회교육충책략，개량고산、감병품충무운갱7호적조문협고병항성。재련속회교화자교과정중，이용여Stv-bi긴밀련쇄적SCAR표기진행근종검측，병결합조문협고병전간항성감정이급농예성상적계통선택，장항성기인Stv-bi전이도무운갱7호중。채용분자표기보조선택，종BC3 F3세대중사선출14개구유Stv-bi순합기인형적개량주계。기종합성상여무운갱7호십분상근，유적성상심지우우무운갱7호。이용정향회교화분자표기보조선택상결합적기술체계，가현저제고항성개량적예견성화준학성，축단육충년한、가쾌육충진정。신육성적무운갱7호항성개량계，위조문협고병항성육충제공료중요적유전자원。
To develop new germplasms resistant to rice stripe disease ( RSV) ,the molecular marker-assisted se-lection(MAS)strategy was applied to improve the resistance of Wuyunjing 7.As the donor parent of resistance gene Stv-b i ,the japonica rice Kantou 194 with high quality was used to improve the RSV resistance of Wuyunjing 7 with high yield but susceptible to RSV by the breeding strategy of directional backcross .In continuous backcross and selfcross generations ,the SCAR( Sequence characterized amplified region ) marker tightly linked with Stv-bi was used for MAS,by combining with the identifications of field resistance to RSV and agronomic traits analysis .And finally, resistance gene was successfully transferred into Wuyunjing 7 .14 improved rice lines carrying the disease-resistance gene were obtained from BC 3 F3 generation by molecular marker selection .These lines were the same as and even better than donor parent in comprehensive characters ,which retained the characteristics of high yield and enhanced obviously the resistance to rice stripe virus .Utilization of technology systems intergrating directional backcross and MAS,the predictability,accuracy and efficiency of breeding for disease resistance could be improved prominently . Meanwhile,these RSV-resistant rice lines,newly developed could provided important genetic resources for the future resistance breeding .