CAJ | 학술논문

腐殖质呼吸是一种新型微生物能量代谢方式，菌种资源研究成为腐殖质呼吸的研究重点。利用厌氧富集分离方法，从木薯堆肥土壤中获得1株具有腐殖质还原特性的兼性厌氧菌株，编号为HN02。 HN02为革兰氏阳性菌，细胞为短杆状，菌落呈黄色，表面光滑，边缘整齐；最适生长盐度为1％，最适生长温度为30℃，最适生长pH值7．5～8．5；可利用麦芽糖作为碳源，明胶液化和硝酸盐还原为阴性；16S rDNA基因序列分析结果表明：菌株HN02与天花板节杆菌亲缘关系最近，同源性达99．4％；HN02能在以葡萄糖作为电子供体，以AQDS为唯一电子受体的厌氧环境下将AQDS还原为AH2 QDS，还原率达40％。菌株HN02是1株具有腐殖质还原特性的天花板节杆菌，首次报道该菌属菌株具有腐殖质还原特性，为完善腐殖质还原特性的微生物系统提供理论基础。
부식질호흡시일충신형미생물능량대사방식，균충자원연구성위부식질호흡적연구중점。이용염양부집분리방법，종목서퇴비토양중획득1주구유부식질환원특성적겸성염양균주，편호위HN02。 HN02위혁란씨양성균，세포위단간상，균락정황색，표면광활，변연정제；최괄생장염도위1％，최괄생장온도위30℃，최괄생장pH치7．5～8．5；가이용맥아당작위탄원，명효액화화초산염환원위음성；16S rDNA기인서렬분석결과표명：균주HN02여천화판절간균친연관계최근，동원성체99．4％；HN02능재이포도당작위전자공체，이AQDS위유일전자수체적염양배경하장AQDS환원위AH2 QDS，환원솔체40％。균주HN02시1주구유부식질환원특성적천화판절간균，수차보도해균속균주구유부식질환원특성，위완선부식질환원특성적미생물계통제공이론기출。
The humus respiration is a new way of microbial energy metabolism .And the research about strains resources becomes the focus point .A facultative anaerobic humus-reducing bacterium ,designated HN02,was isola-ted from cassava dregs composting .Strain HN02 is a gram-positive,short rod-shaped,gelatin hydrolysis and nitrate reduction-negative bacterium .Optimal growth salt was 1%( NaCl);optimal growth temperature was 30℃and opti-mal pH was at 7.5-8.5.Analysis of 16S rDNA of HN02 indicated that the closest phylogenetic relative among the valid species was Arthrobacter tecti,with 99.4%16S rDNA similarity.Strain HN02 can utilize AQDS via anaerobic enrichment procedure with glucose as the electron donor and AQDS as the sole terminal electron acceptor .The re-duction ratio of AQDS was 40%.Thus,the humus-reducing of Arthrobacter tecti HN02 is here reported for the first time and it can provides the theoretical basis for microbial systems with characteristics of humus reduction .