华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2013年
4期
158-162
,共5页
李翠芳%刘连涛%孙红春%李存东
李翠芳%劉連濤%孫紅春%李存東
리취방%류련도%손홍춘%리존동
一氧化氮%棉花幼苗%氧化损伤%保护酶
一氧化氮%棉花幼苗%氧化損傷%保護酶
일양화담%면화유묘%양화손상%보호매
Nitric oxide%Cotton seedling%Oxidative damage%Protective enzyme
以子叶全展期的棉花幼苗为材料,在1/4 Hoagland营养液中添加不同浓度,0,10,50,100,200μmol/L SNP (硝普钠)处理棉花幼苗,研究外源NO对棉花幼苗叶片和根系氧化损伤和保护酶活性的影响。结果表明:外源NO供体SNP处理使棉苗叶片和根系MDA含量和O·-2产生速率显著增加,可溶性蛋白含量(叶片)显著降低,SOD、POD活性增加,但是CAT活性下降。10~100μmol/L SNP处理间差异不显著。各生理指标之间差异优先表现在叶片中。结果表明,外源NO在一定浓度范围内抑制棉花幼苗的生长。
以子葉全展期的棉花幼苗為材料,在1/4 Hoagland營養液中添加不同濃度,0,10,50,100,200μmol/L SNP (硝普鈉)處理棉花幼苗,研究外源NO對棉花幼苗葉片和根繫氧化損傷和保護酶活性的影響。結果錶明:外源NO供體SNP處理使棉苗葉片和根繫MDA含量和O·-2產生速率顯著增加,可溶性蛋白含量(葉片)顯著降低,SOD、POD活性增加,但是CAT活性下降。10~100μmol/L SNP處理間差異不顯著。各生理指標之間差異優先錶現在葉片中。結果錶明,外源NO在一定濃度範圍內抑製棉花幼苗的生長。
이자협전전기적면화유묘위재료,재1/4 Hoagland영양액중첨가불동농도,0,10,50,100,200μmol/L SNP (초보납)처리면화유묘,연구외원NO대면화유묘협편화근계양화손상화보호매활성적영향。결과표명:외원NO공체SNP처리사면묘협편화근계MDA함량화O·-2산생속솔현저증가,가용성단백함량(협편)현저강저,SOD、POD활성증가,단시CAT활성하강。10~100μmol/L SNP처리간차이불현저。각생리지표지간차이우선표현재협편중。결과표명,외원NO재일정농도범위내억제면화유묘적생장。
Cotyledon full extension of the cotton seedling as material ,in the 1/4 Hoagland nutrient solution with different concentration of SNP( 0,10,50,100,200 μmol/L)processing cotton seedling,study of oxidative damage and activities of protective enzymes of exogenous nitric oxide on cotton seedling leaves and roots .The results show that:with the treatment of SNP ,the content of MDA and the generation rate of O ·-2 of cotton seedling leaves and roots increased significantly ,the content of soluble protein of leaves decreased significantly , SOD and POD activity in-creased ,while CAT activity decreased .The difference under 10-100 μmol/L SNP processing were not remarkable between treatments .The difference of physiological indexes between treatments was priority performance in leaves . These results suggest that exogenous nitric oxide in certain concentration range inhibit cotton seedling growth .