CAJ | 학술논문

为了探索前期不同烟株营养状况协同成熟期氮素调亏对烟叶质体色素代谢影响，筛选出表达水平与氮素水平密切相关的色素转化降解基因。运用盆栽试验，在前期不同氮素营养水平协同后期同量调亏的条件下对烤烟NC297的质体色素含量、成熟期光合速率、叶片超微结构和相关基因表达进行了研究。结果表明：移栽后30～60 d，各时期质体色素含量随着施氮量的增加而增加。随着施氮量的增加，成熟期烟株净光合速率增加；细胞内的嗜锇颗粒、叶绿体和类囊体片层数量增加，衰老特异性半胱氨酸蛋白酶基因CP1的表达逐渐减弱，细胞衰老降解速度减慢。高氮处理N1的脱镁叶绿酸a加氧酶基因PAO在发育后期表达水平高于其他处理；ζ-胡萝卜素脱氢酶基因ZDS、类胡萝卜素裂解双氧合酶基因CCDs和9-顺式环氧类胡萝卜素双加氧酶基因NCED对施氮水平反应敏感，随着施氮量增加其表达高峰向后推移。综上，基因PAO、ZDS、CCDs和NCED表达高峰的出现时间可作为反映后期氮素调亏条件下烟株氮素营养状况是否适宜的有效指标。
위료탐색전기불동연주영양상황협동성숙기담소조우대연협질체색소대사영향，사선출표체수평여담소수평밀절상관적색소전화강해기인。운용분재시험，재전기불동담소영양수평협동후기동량조우적조건하대고연NC297적질체색소함량、성숙기광합속솔、협편초미결구화상관기인표체진행료연구。결과표명：이재후30～60 d，각시기질체색소함량수착시담량적증가이증가。수착시담량적증가，성숙기연주정광합속솔증가；세포내적기철과립、협록체화류낭체편층수량증가，쇠로특이성반광안산단백매기인CP1적표체축점감약，세포쇠로강해속도감만。고담처리N1적탈미협록산a가양매기인PAO재발육후기표체수평고우기타처리；ζ-호라복소탈경매기인ZDS、류호라복소렬해쌍양합매기인CCDs화9-순식배양류호라복소쌍가양매기인NCED대시담수평반응민감，수착시담량증가기표체고봉향후추이。종상，기인PAO、ZDS、CCDs화NCED표체고봉적출현시간가작위반영후기담소조우조건하연주담소영양상황시부괄의적유효지표。
In order to explore the influence of different nitrogen levels and equal nitrogen deficiency at maturing stage on plastid pigment metabolism in flue-cured tobacco and select the key genes closely related to nitrogen levels of plastid pigment transform and degradation metabolism ,pot experiment was conducted .The ultrastructure ,net pho-tosynthetic rate and the plastid pigment contents were compared in flue-cured tobacco NC297 .The expression levels of several key genes related to plastid pigment metabolism were also analyzed .The results showed that on the 30 d and 60 d after transplanting ,the contents of plastid pigment in tobacco leaves increased with the rising of the nitro-gen level .The net photosynthetic rates were consistent with the variation of plastid pigment contents .The contents of osmiophilic granule and thylakoid lamella of chloroplast were improved , while the aging and degradation rates de-layed with the rising of nitrogen level during the maturity period .The expression of nicotiana tabacum senescence-specific cysteine protease ( CP1 ) was gradually decreased .The expression of pheophorbide a monooxygenase ( PAO) in super high nitrogen treatment N 1 was siginificantly higher than other treatments at the end of the mature stage.Expression peaks of ζ-carotene desaturase (ZDS),carotenoid cleavage dioxygenases,CCDs and 9-cis-epoxy carotenoid dioxygenase (NCED) were postponed with the rising of the nitrogen level .In conclusion,the timing of expression peak of gene PAO,ZDS,CCDs and NCED can be used as the index for the evaluation of nitrogen nutri-tion conditions of the tobacco plants .