CAJ | 학술논문

以高羊茅为研究对象，采用不同PEG模拟干旱处理（CK、10％PEG、0．1 mmol/L SNP＋10％PEG、1．0 mmol/L SNP＋10％PEG等）方法，就外源NO供体SNP对高羊茅种子的萌发、幼苗生长及抗逆性的影响进行了探讨。结果表明，在PEG模拟干旱胁迫下，高羊茅种子的发芽率、发芽势、发芽指数、活力指数和幼苗叶绿素含量较对照呈下降趋势，而幼苗丙二醛（ MDA）、脯氨酸、可溶性糖、超氧阴离子（ O·-2）和过氧化氢（ H2 O2）含量呈上升趋势，超氧化物歧化酶（ SOD）和过氧化物酶（ POD）活性则呈现先升高后降低的趋势。上述结果说明PEG模拟的干旱胁迫，使得高羊茅在种子萌发及幼苗生长过程中遭受逆境胁迫，且生长发育受到显著抑制。经外源NO供体SNP处理后，模拟干旱胁迫下的高羊茅种子发芽率、发芽势、发芽指数、活力指数和幼苗叶片叶绿素、脯氨酸、可溶性糖含量及SOD、POD、CAT活性均显著升高，而幼苗叶片MDA、O·-2和H2 O2含量显著下降，说明外源NO供体SNP处理后使PEG模拟干旱胁迫下高羊茅的生长发育得到了促进，减轻了干旱胁迫对高羊茅造成的伤害，提高了植株的整体抗旱性。通过对比几种不同SNP浓度，结果说明0．1 mmol/L SNP对PEG模拟干旱胁迫下高羊茅种子萌发及幼苗的保护效应较为显著。
이고양모위연구대상，채용불동PEG모의간한처리（CK、10％PEG、0．1 mmol/L SNP＋10％PEG、1．0 mmol/L SNP＋10％PEG등）방법，취외원NO공체SNP대고양모충자적맹발、유묘생장급항역성적영향진행료탐토。결과표명，재PEG모의간한협박하，고양모충자적발아솔、발아세、발아지수、활력지수화유묘협록소함량교대조정하강추세，이유묘병이철（ MDA）、포안산、가용성당、초양음리자（ O·-2）화과양화경（ H2 O2）함량정상승추세，초양화물기화매（ SOD）화과양화물매（ POD）활성칙정현선승고후강저적추세。상술결과설명PEG모의적간한협박，사득고양모재충자맹발급유묘생장과정중조수역경협박，차생장발육수도현저억제。경외원NO공체SNP처리후，모의간한협박하적고양모충자발아솔、발아세、발아지수、활력지수화유묘협편협록소、포안산、가용성당함량급SOD、POD、CAT활성균현저승고，이유묘협편MDA、O·-2화H2 O2함량현저하강，설명외원NO공체SNP처리후사PEG모의간한협박하고양모적생장발육득도료촉진，감경료간한협박대고양모조성적상해，제고료식주적정체항한성。통과대비궤충불동SNP농도，결과설명0．1 mmol/L SNP대PEG모의간한협박하고양모충자맹발급유묘적보호효응교위현저。
The seed germination ,seedling growth and stress resistance of Festuca arundinacea were discussed in this paper as the different treatment ( CK,10% PEG,0.1 mmol/L SNP +10% PEG,1.0 mmol/L SNP +10%PEG) were carried out in the experiments .The results showed that Festuca arundinacea seed germination rate ,ger-minating energy ,germination index ,vigor index and seedling chlorophyll content and catalase ( CAT) activities were downward trend,but the content of malondialdehyde (MDA),proline,soluble sugar,superoxide anion(O·-2 ) and hy-drogen peroxide ( H2 O2 ) of seedling leaves increased ,the activities of superoxide dismutase ( SOD) and peroxidase ( POD) increased firstly and then decreased under the simulated drought stress .The results also showed that the growth and development of Festuca arundinacea were inhibited significantly under the simulated drought stress and the seedling suffered from drought stress .As being treated with the exogenous NO donor SNP ,the Festuca arundina-cea seed germination rate ,germinating energy ,germination index ,vigor index ,and the leaves′content of chlorophyll , proline and soluble sugar,the activities of SOD,POD and CAT increased,the content of MDA,O·-2 and H2O2 de-clined .The results also showed that the growth and development of Festuca arundinacea were promoted ,injury miti-gated when the seedlings enduring the PEG simulated drought stress being treated with the exogenous NO donor SNP.So it can be concluded that when the seedlings being treated with exogenous NO donor SNP , the protective effect is significant when the seedlings suffered from the 10%PEG solution simulated drought stress .