CAJ | 학술논문

为了提高玉米中赖氨酸含量，利用基因枪技术将Sb401基因导入玉米杂交组合HiⅡAB的愈伤组织中，在添加Bialaphos浓度为1．5，3．0 mol/L的选择培养基上进行2次筛选，然后分化再生植株，对所获得的植株进行PCR检测和免疫试纸条检测。结果显示，经过2次筛选获得58株再生植株，PCR检测呈阳性的植株有38株，将PCR阳性植株再通过免疫试纸条检测，确定bar蛋白得到表达的植株有31株，结果表明，共获得31株含有目的基因的玉米植株。
위료제고옥미중뢰안산함량，이용기인창기술장Sb401기인도입옥미잡교조합HiⅡAB적유상조직중，재첨가Bialaphos농도위1．5，3．0 mol/L적선택배양기상진행2차사선，연후분화재생식주，대소획득적식주진행PCR검측화면역시지조검측。결과현시，경과2차사선획득58주재생식주，PCR검측정양성적식주유38주，장PCR양성식주재통과면역시지조검측，학정bar단백득도표체적식주유31주，결과표명，공획득31주함유목적기인적옥미식주。
The purpose of this study was to improve the lysine content in corn ,Sb401 gene was introdued into imma-ture embryo callus using particle bombardment .Regeneration into plants by selection for 1.5,3.0 mol/L bialaphos resist-ance.PCR detection in the obtained by plants and protein dipsticks testing .The results show that 38 transgenic plants were identified by PCR from 58 regenerated transformed plants obtained by selection for bialaphos resistance ,31 transgen-ic plants was identified by test trip .It turned out that 31 transgenic plants has introduced target gene .