CAJ | 학술논문

运用RT-PCR和PCR技术克隆基因，应用DNAStar对获得的cDNA及推测的氨基酸序列进行分析。从合作猪脾脏组织中克隆IFN-γcDNA ORF全长501 bp，共编码166个氨基酸，N端具有由23个氨基酸组成的信号肽，该蛋白预测的分子质量为19．4185 kDa，等电点为9．54，合作猪IFN-γ核苷酸序列上共发现9个磷酸化位点，分别为Ser （7）、Thr（1）、Tyr（1）；2个N-糖基化位点，分别在第39位和第106位Asn处。序列比较结果表明，合作猪IFN-γ基因序列与藏猪、印度猪种、内江猪、牛、狗的同源性较高，与人类和鸡的同源性较低。对于小分子蛋白质和多肽，有义突变和无义突变对于同源性及系统进化的分析影响较大，应结合核苷酸和氨基酸2个层面综合分析，并且IFN-γ和高原低氧作用于机体产生的NO，能有效地抑制寄生虫病的发生。
운용RT-PCR화PCR기술극륭기인，응용DNAStar대획득적cDNA급추측적안기산서렬진행분석。종합작저비장조직중극륭IFN-γcDNA ORF전장501 bp，공편마166개안기산，N단구유유23개안기산조성적신호태，해단백예측적분자질량위19．4185 kDa，등전점위9．54，합작저IFN-γ핵감산서렬상공발현9개린산화위점，분별위Ser （7）、Thr（1）、Tyr（1）；2개N-당기화위점，분별재제39위화제106위Asn처。서렬비교결과표명，합작저IFN-γ기인서렬여장저、인도저충、내강저、우、구적동원성교고，여인류화계적동원성교저。대우소분자단백질화다태，유의돌변화무의돌변대우동원성급계통진화적분석영향교대，응결합핵감산화안기산2개층면종합분석，병차IFN-γ화고원저양작용우궤체산생적NO，능유효지억제기생충병적발생。
IFN-γgene was cloned by RT-PCR,the cDNA was cloned and sequenced which structure of its en-coding protein were predicted by using DNAStar software .Sequence analysis results showed that the IFN-γgene ORF was 501 bp and encoded 166 amino acids,including a signal peptide of 23 amino acids(aa) at the N terminal. Relative molecular weight of the encoding protein was 19.418 5 kDa,and the isoelectric point ( pI) was 9.54. There were 9 phosphorylation sites including 7 Sers,1 Thr and 1 Tyr.In addition,2 N-glycosylation sites were found at Asn about 39 th and 106 th in the line .Sense mutation and nonsense mutation in small protein or polypeptide have large influence on the analysis of system evolution and percent identity ,so it should be multianalysis on nucleotide and amine acid .IFN-γand plateau and hypoxia induced body to produce NO .It could inhibit parasitic disease ef-fectively.