CAJ | 학술논문

本实验克隆了果蝇与人源P-TEFb(positive transcription elongation factor)组成亚基CycT、CDK9 C末端分别带有Flag、HA标签的真核表达质粒，并在293细胞中验证了克隆所得质粒的有效表达。使用免疫共沉淀方法证实了两个种属 P-TEFb 组成亚基间结构的相似性；借助 HIV-luciferase与Rellina的双荧光报告系统证实了两个种属P-TEFb 组成亚基间功能上的相似性。
본실험극륭료과승여인원P-TEFb(positive transcription elongation factor)조성아기CycT、CDK9 C말단분별대유Flag、HA표첨적진핵표체질립，병재293세포중험증료극륭소득질립적유효표체。사용면역공침정방법증실료량개충속 P-TEFb 조성아기간결구적상사성；차조 HIV-luciferase여Rellina적쌍형광보고계통증실료량개충속P-TEFb 조성아기간공능상적상사성。
In this study eukaryotic expression plasmid of Drosophila and human P-TEFb's subunit gene CDK9 and CycT was cloned,which each of the subunit has a Flag or HA tag in the C terminal respec-tively.Also was proved well express in the 2 9 3 Cell line.With the method of immunoprecipitation Dro-sophila P-TEFb and human P-TEFb subunits'structure similarity was well confirmed.By means of dual-luciferase report system of HIV-luciferase and Rellina,P-TEFb subunits'function similarity of the two species was also well affirmed.