CAJ | 학술논문

以拟南芥油菜素内酯受体BRI1基因序列设计同源简并引物,利用同源克隆技术,克隆了甘蓝型油菜中编码油菜素内酯受体基因全长 cDNA 序列,命名为BnBRI1(GenBank：JX871217),该基因开放阅读框(ORF)大小为3591 bp,与拟南芥中BRI1基因(GenBank：NM_120100)相似性为94.21%,编码1196个氨基酸,与拟南芥中BRI 编码的氨基酸相似性为89.23%.采用实时定量PCR分析表明,BnBRI1基因在根、茎、叶中都有表达,但表达量存在显著差异.在两叶一心期和花期的根中表达量最高,四叶一心期和抽薹期的叶中表达量最高.
이의남개유채소내지수체BRI1기인서렬설계동원간병인물,이용동원극륭기술,극륭료감람형유채중편마유채소내지수체기인전장 cDNA 서렬,명명위BnBRI1(GenBank：JX871217),해기인개방열독광(ORF)대소위3591 bp,여의남개중BRI1기인(GenBank：NM_120100)상사성위94.21%,편마1196개안기산,여의남개중BRI 편마적안기산상사성위89.23%.채용실시정량PCR분석표명,BnBRI1기인재근、경、협중도유표체,단표체량존재현저차이.재량협일심기화화기적근중표체량최고,사협일심기화추대기적협중표체량최고.
Homologous degenerented primers were designed based on the sequence of the brassinolide re-ceptor gene BRI1 in Arabidopsis thaliana.The method of homologous clone was used to clone and ana-lyze a full-length cDNA of the brassinolide receptor gene in Brassica napus L.which was named Bn-BRI1 (GenBank:JX871217 ).The 3591bp-length of the whole sequence of the open reading frame (ORF)of BnBRI had 94.21% similarity with Arabidopsisthaliana BRI (GenBank:NM_120100).For the putative protein,the protein of BnBRI1 contained 1196 amino acids having 89.23%similarity with the BRI1 protein inArabidopsis thaliana.Then the expression of BnBRI1 in different tissues of the dif-ferent development stages in B.napus L.was analyzed using real-time polymerase chain reaction.This result showed that the expression ofBnBRI1 existed in different tissues of the different development pe-riods,but the amount of expression varied,highest in the root of two-leaf and flower period and in the leaf of four-leaf period and bolting period.