CAJ | 학술논문

目的探讨他莫西芬对胶质瘤细胞系BT-325的抑制作用及其可能机制。方法应用Cell Counting Kit-8（CCK-8）试剂盒法检测细胞活性，观察1、3、10、30、100μmol/L他莫西芬对胶质瘤细胞系BT-325活性的影响，并检测雌激素α受体激动剂（ PPT ）、β受体激动剂（ DPN ）和雌激素受体（ ER ）抑制剂（ICI182，780）对他莫西芬作用的影响；免疫荧光法检测BT-325中ER的表达。结果3μmol/L他莫西芬使BT-325细胞皱缩，细胞活性下降，存活率降至83.97％，并随着他莫西芬浓度的升高，细胞皱缩和活性下降也变得更加明显，当浓度达到30μmol/L时，细胞存活率仅为25.73％。 PPT、DPN和ICI182，780均不影响10μmol/L他莫西芬对BT-325的作用，并且免疫荧光结果显示BT-325细胞不存在ER的表达。结论他莫西芬对胶质瘤细胞系BT-325活性具有抑制作用，而这种作用与ER途径无关。
목적탐토타막서분대효질류세포계BT-325적억제작용급기가능궤제。방법응용Cell Counting Kit-8（CCK-8）시제합법검측세포활성，관찰1、3、10、30、100μmol/L타막서분대효질류세포계BT-325활성적영향，병검측자격소α수체격동제（ PPT ）、β수체격동제（ DPN ）화자격소수체（ ER ）억제제（ICI182，780）대타막서분작용적영향；면역형광법검측BT-325중ER적표체。결과3μmol/L타막서분사BT-325세포추축，세포활성하강，존활솔강지83.97％，병수착타막서분농도적승고，세포추축화활성하강야변득경가명현，당농도체도30μmol/L시，세포존활솔부위25.73％。 PPT、DPN화ICI182，780균불영향10μmol/L타막서분대BT-325적작용，병차면역형광결과현시BT-325세포불존재ER적표체。결론타막서분대효질류세포계BT-325활성구유억제작용，이저충작용여ER도경무관。
Objective To study the inhibitory effects of tamoxifen on human BT-325 glioma cell in vitro. Methods Cultured BT-325 glioma cells were treated with different concentration of tamoxifen ,their celluar activity was evaluated by CCK-8 assay.The effect of various concentrations of α-estrogen receptor(ER)agonist PPT,β-ER agonist DPN,or ER antagonist ICI182,780 alone or with tamoxifen (10 μm) on BT-325 viability was measured by CCK-8 assay.The expressions of ER were examined by immumofluorescence method .Results Tamoxifen induced BT-325 glioma cells shedding and inhibited cell viability in a dose-dependent manner .ER agonists DPN and PPT or ER antagonists ICI182,780 neither caused influence on BT-325 cell viability,nor blocked the effects of tamoxifen on cell viability of BT-325 cells.There was no ER on BT-325 cells observed by laser scanning confocal microscope . Conclusions Tamoxifen can inhibit BT-325 glioma cells independently of ER in vitro.