CAJ | 학술논문

目的探讨芍药苷（paeoniflorin）对胶原诱导型大鼠关节炎（CIA）滑膜增殖和治疗的影响。方法采用完全弗氏佐剂和Ⅱ型胶原诱导大鼠CIA模型，计数方法测定多发性关节炎指数（AI），称重法比较大鼠的体重变化，HE染色法光镜观察关节病理形态学变化，酶联免疫吸附试验（ ELISA ）检测巨噬细胞分泌的白细胞介素-6（IL-6）、肿瘤坏死因子（TNF）-α，免疫组化检测滑膜细胞凋亡水平。结果芍药苷对CIA大鼠的关节炎指数和关节病理评分都有改善，芍药苷组的各炎症因子的表达较模型组降低，芍药苷治疗后滑膜细胞凋亡较对照组增加，差异均有统计学意义（ P＜0.05）。结论芍药苷可以增加CIA大鼠关节炎滑膜细胞凋亡，降低CIA大鼠的关节炎指数和炎症因子含量。
목적탐토작약감（paeoniflorin）대효원유도형대서관절염（CIA）활막증식화치료적영향。방법채용완전불씨좌제화Ⅱ형효원유도대서CIA모형，계수방법측정다발성관절염지수（AI），칭중법비교대서적체중변화，HE염색법광경관찰관절병리형태학변화，매련면역흡부시험（ ELISA ）검측거서세포분비적백세포개소-6（IL-6）、종류배사인자（TNF）-α，면역조화검측활막세포조망수평。결과작약감대CIA대서적관절염지수화관절병리평분도유개선，작약감조적각염증인자적표체교모형조강저，작약감치료후활막세포조망교대조조증가，차이균유통계학의의（ P＜0.05）。결론작약감가이증가CIA대서관절염활막세포조망，강저CIA대서적관절염지수화염증인자함량。
Objective To investigate the effects of paeoniflorin on the proliferation of synovinm in rats with collagenⅡinduced arthritis .Methods SD rats were injected with typeⅡcollagen and complete Freund′s adjuvant ( CFA) .The inflammation of CIA rats was evaluated by arthritis index and weights ,also histopathology of joints .The levels of IL-6,TNF-αin serum were measured by enzyme-linked immuno-absorbent assay(ELISA)respectively.The synoviocytes apoptosis index were assayed by immunohistochemistry .Results The administration of Pae inhibited the inflammatory response and reduced the AI of rats and restored the weights of CIA rats .Pae reduced the levels of IL-6,TNF-αin serum and increase the apoptosis index in rats with collagenⅡ(P<0.05).Conclusions Pae have anti-inflammatory effects ,it can enhance apoptosis index of synoviocytes and reduce AI of CIA rats .