CAJ | 학술논문

目的观察大鼠深Ⅱ度烫伤后72 h内创面自噬及凋亡的表达规律，探讨其与深Ⅱ度创面早期加深之间的关系。方法 Wistar大鼠背部10％TBSA深Ⅱ度烫伤，观察烫伤后1 h、6 h、12 h、24 h、48 h、72 h创面组织自噬标志蛋白LC3、Beclin-1的表达及变化规律；TUNEL法检测创面凋亡水平的变化规律；HE染色及Masson染色显示创面组织形态和创面深度的变化。结果烫伤后创面组织LC3、Beclin-1蛋白水平持续下降，至伤后24 h达到最低，此后稍有升高，但仍远低于正常水平；伤后创面组织TUNEL阳性细胞持续增多，至伤后48 h达到最高，此后稍有下降，但仍远高于正常水平；创面组织真皮深层自噬被激活；烫伤后创面深度随着时间的推移进行性加深。结论大鼠深Ⅱ度烫伤后早期创面组织自噬减少，凋亡增加，可能是参与创面加深的因素；真皮深层自噬的激活，可能是一种保护机制。
목적관찰대서심Ⅱ도탕상후72 h내창면자서급조망적표체규률，탐토기여심Ⅱ도창면조기가심지간적관계。방법 Wistar대서배부10％TBSA심Ⅱ도탕상，관찰탕상후1 h、6 h、12 h、24 h、48 h、72 h창면조직자서표지단백LC3、Beclin-1적표체급변화규률；TUNEL법검측창면조망수평적변화규률；HE염색급Masson염색현시창면조직형태화창면심도적변화。결과탕상후창면조직LC3、Beclin-1단백수평지속하강，지상후24 h체도최저，차후초유승고，단잉원저우정상수평；상후창면조직TUNEL양성세포지속증다，지상후48 h체도최고，차후초유하강，단잉원고우정상수평；창면조직진피심층자서피격활；탕상후창면심도수착시간적추이진행성가심。결론대서심Ⅱ도탕상후조기창면조직자서감소，조망증가，가능시삼여창면가심적인소；진피심층자서적격활，가능시일충보호궤제。
Objective To observe the changes of autophagy and apoptosis in wounds after deep second -degree burn in rats and discuss their roles in burn wound progression .Methods The Wistar rats were subjected to 10%second-degree burn injury .The expression of LC3 and Beclin-1 in the burn wounds was observed 1 h,6 h,12 h, 24 h, 48 h and 72 h postburn respectively .Apoptosis in the burn wound was determined by Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling assay ( TUNEL ) .Pathologic changes were assessed by using HE and Masson staining .Results The LC3 and Beclin-1 protein level in burn wounds continuously decreased in 24 h postburn,and then began to increase but still could not reach their normal level at 72 h postburn.The TUNEL positive cells in wounds increased continuously in 48 h postburn ,and then began to decrease but still could not reach normal level at 72 h postburn.Enhanced autophagy was observed in the deep dermal layer of burn wounds . Conclusions At the early stage after burn ,autophagy was suppressed and apoptosis was increased in wounds which may be involved in burn wound progression .The enhanced autophagy in the deep dermal layer of burn wounds might be a protective factor in burn wound progression .