CAJ | 학술논문

目的检测糖尿病性勃起功能障碍（ ED）大鼠阴茎海绵体组织中内皮型一氧化氮合成酶运输转导物（NOSTRIN）的表达。方法将12周龄雄性SD大鼠20只随机分为2组，对照组8只，糖尿病组12只，链脲佐菌素诱导糖尿病性ED模型，8周后检测两组大鼠阴茎海绵体内压，并取阴茎海绵体组织行real time RT-PCR、Western blot、免疫组化染色测定NOSTRIN、eNOS的表达。结果糖尿病组大鼠阴茎海绵体内压比造模前明显降低（P＜0.01），而对照组无明显差异。与对照组比较，糖尿病组NOSTRIN表达升高，eNOS表达降低（ P＜0.01）。结论 NOSTRIN在糖尿病性ED大鼠阴茎海绵体中表达升高，eNOS表达降低。
목적검측당뇨병성발기공능장애（ ED）대서음경해면체조직중내피형일양화담합성매운수전도물（NOSTRIN）적표체。방법장12주령웅성SD대서20지수궤분위2조，대조조8지，당뇨병조12지，련뇨좌균소유도당뇨병성ED모형，8주후검측량조대서음경해면체내압，병취음경해면체조직행real time RT-PCR、Western blot、면역조화염색측정NOSTRIN、eNOS적표체。결과당뇨병조대서음경해면체내압비조모전명현강저（P＜0.01），이대조조무명현차이。여대조조비교，당뇨병조NOSTRIN표체승고，eNOS표체강저（ P＜0.01）。결론 NOSTRIN재당뇨병성ED대서음경해면체중표체승고，eNOS표체강저。
Objective To investigate the expression change of endothelial nitric oxide synthase traffic inducer(NOSTRIN)in the cavernous tissues of diabetes mellitus erectile dysfunction (DMED)rats.Methods Twenty male Sprague-Dawley rats were divided into Groups A(normal control,n=8)and B(DM group,n=12),the diabetic models were made by intraperitoneal injection of strepozocin ( STZ) .Eight weeks later ,the erectile function of the rats was assessed through measuring the mean arterial pressure (MAP)and intracavernous pressure(ICP).The expression changes of NOSTRIN and eNOS were detected in cavernous tissues by real time reverse transcription PCR ,Western blot and immunohistochemical staining .Results ICP of group B was significantly lower than that of group A ( P<0.01 ) .Compared with group A , the expression of NOSTRIN was markedly increased , the expression of eNOS significantly decreased ( P<0.01 ) .Conclusions The expression of NOSTRIN is increased and the expression of eNOS is decreased in the cavernous tissues of DMED rats .