CAJ | 학술논문

目的研究硫化氢（ H2 S）对七叶皂苷钠（ SA）细胞毒性的保护作用。方法培养HK-2细胞分别建立H2 S处理[培养基中添加H2 S的供体硫氢化钠（ NaHS ）]和 H2 S 关键合成酶胱硫醚β合成酶（ CBS）、胱硫醚γ裂解酶（ CSE）的抑制剂炔丙基甘氨酸（ PPG）处理的实验模型。 CCK-8实验检测细胞存活率；流式细胞凋亡实验检测细胞凋亡率。结果 SA 处理 HK-2细胞24 h 后，细胞存活率为（48.21±3.57）％，细胞凋亡率为（40.8±1.41）％。同时给予SA和NaHS处理，HK-2细胞存活率[（74.35±3.62）％]升高，凋亡率[（17.7±0.55）％]降低（ P＜0.01）；相反，同时给予SA和PPG处理，对HK-2细胞的毒性作用增强，细胞存活率[（19.67±2.31）％]降低，细胞凋亡率[（76.91±2.36）％]升高（P＜0.01）。结论 H2S能对抗SA对HK-2细胞的毒性作用，抑制细胞凋亡。
목적연구류화경（ H2 S）대칠협조감납（ SA）세포독성적보호작용。방법배양HK-2세포분별건립H2 S처리[배양기중첨가H2 S적공체류경화납（ NaHS ）]화 H2 S 관건합성매광류미β합성매（ CBS）、광류미γ렬해매（ CSE）적억제제결병기감안산（ PPG）처리적실험모형。 CCK-8실험검측세포존활솔；류식세포조망실험검측세포조망솔。결과 SA 처리 HK-2세포24 h 후，세포존활솔위（48.21±3.57）％，세포조망솔위（40.8±1.41）％。동시급여SA화NaHS처리，HK-2세포존활솔[（74.35±3.62）％]승고，조망솔[（17.7±0.55）％]강저（ P＜0.01）；상반，동시급여SA화PPG처리，대HK-2세포적독성작용증강，세포존활솔[（19.67±2.31）％]강저，세포조망솔[（76.91±2.36）％]승고（P＜0.01）。결론 H2S능대항SA대HK-2세포적독성작용，억제세포조망。
Objective To investigate the effects of H 2 S protecting HK-2 cell from the toxic effects of aescin.Methods HK-2 Epithelial cell line of human renal proximal tubule was cultivated in KSFM medium with 10%fetal bovine serum ,NaHS as supplier of H 2 S and DL-propargylglycine ( PPG) as the inhibitor of cystathionine γlyase respectively .Cell viability and apoptosis were detected by cell vitality test and flow cytometry . Results Survival rate was(48.21 ±3.57)% and apoptosis rate was(40.8 ±1.41)% when the group was treated with SA 24 hours.Cell survival rate[(74.35 ±3.62)%]was increased and apoptosis rate[(40.8 ±1.41)%]was reduced compared with control group when SA and NaHS were added into medium (P<0.01).However,there were opposite effect when the group was treated with PPG and SA ( P<0.01 ) .Conclusions H2 S can reverse the toxic effects which aescin affect HK-2 cell.