CAJ | 학술논문

目的探讨印记基因PHLDA2在骨肉瘤放疗疗效评估中的意义。方法采用免疫组织化学S-P法和RT-PCR方法在36例放射治疗后的骨肉瘤组织及其配对的癌旁组织中检测PHLDA2分子的表达，并结合临床病例参数及辐射敏感性探讨PHLDA2表达的意义。结果放射治疗后的骨肉瘤组织中PHLDA2分子mRNA和蛋白的表达一致，明显低于配对的癌旁组织，差异具有统计学意义（ P＜0.05）；辐射缓解组中PHLDA2的表达阳性率高，与辐射抵抗组差异有统计学意义（ P＜0.05）；但PHLDA2表达与骨肉瘤患者的性别、年龄、病理分型不相关，差异无统计学意义（ P＞0.05）。结论骨肉瘤组织中PHLDA2的表达显著降低，其表达可作为一种骨肉瘤放疗疗效评估的参考指标，值得在临床上推广运用。
목적탐토인기기인PHLDA2재골육류방료료효평고중적의의。방법채용면역조직화학S-P법화RT-PCR방법재36례방사치료후적골육류조직급기배대적암방조직중검측PHLDA2분자적표체，병결합림상병례삼수급복사민감성탐토PHLDA2표체적의의。결과방사치료후적골육류조직중PHLDA2분자mRNA화단백적표체일치，명현저우배대적암방조직，차이구유통계학의의（ P＜0.05）；복사완해조중PHLDA2적표체양성솔고，여복사저항조차이유통계학의의（ P＜0.05）；단PHLDA2표체여골육류환자적성별、년령、병리분형불상관，차이무통계학의의（ P＞0.05）。결론골육류조직중PHLDA2적표체현저강저，기표체가작위일충골육류방료료효평고적삼고지표，치득재림상상추엄운용。
Objective To study imprinted gene PHDLA2 expression for assessment of radiosensitivity in the tissues of human osteosarcoma .Methods Immunohistochemical SP method and RT-PCR method were used to detect the expression of PHLDA2 in 36 cases of osteosarcoma and their paired noncancerous after radiotherapy ,and analyze the relationship between PHLDA 2 expression and clinical parameters and radiosensitivity .Results The mRNA expression of PHLDA2 was consistent with the expression of protein in osteosarcoma ,which was significantly lower than the expression of paired noncancerous ( P <0.05 );meanwhile the positive expressed rate of PHLDA2 in radiosensitive group was significantly higher than that of radioresistant group ( P<0.05 ) .The PHLDA2 expression was not irrelevant with the gender , age and pathological typing of osteosarcoma ( P >0.05 ) .Conclusions The expression of PHLDA2 significantly reduce in osteosarcoma , which play an important role in evaluating the radiosensitivity of osteosarcoma .