CAJ | 학술논문

目的：探讨创伤后应激障碍(PTSD)大鼠杏仁核Akt和mTOR蛋白表达的变化。方法：随机将40只雄性Wistar大鼠分为对照组和模型组，采用改良的单一连续应激方法(SPS & S)建立PTSD大鼠模型，分别采用免疫组织化学染色和Western blot法检测杏仁核p-Akt和p-mTOR蛋白的表达。结果：模型组大鼠杏仁核p-Akt、p-mTOR的相对表达水平显著低于对照组，组间比较差异具有统计学意义(P＜0.05)。结论：PTSD大鼠杏仁核Akt和mTOR磷酸化水平降低，可能与PTSD时杏仁核神经元的凋亡增加有关。
목적：탐토창상후응격장애(PTSD)대서행인핵Akt화mTOR단백표체적변화。방법：수궤장40지웅성Wistar대서분위대조조화모형조，채용개량적단일련속응격방법(SPS & S)건립PTSD대서모형，분별채용면역조직화학염색화Western blot법검측행인핵p-Akt화p-mTOR단백적표체。결과：모형조대서행인핵p-Akt、p-mTOR적상대표체수평현저저우대조조，조간비교차이구유통계학의의(P＜0.05)。결론：PTSD대서행인핵Akt화mTOR린산화수평강저，가능여PTSD시행인핵신경원적조망증가유관。
Objective:To observe the changes of Akt and mTOR expression in amygdala of posttraumatic stress disorder (PTSD) rats. Methods:40 male Wistar rats were randomly divided into control group and model group. Single inescapable electric foot shock after SPS procedures (SPS&S) was used to made PTSD rats’ model. Immunohistochemical staining and Western blot were respectively used to detect the p-Akt and p-mTOR protein expression in amygdala.Results:Compared with control group, the p-Akt and p-mTOR protein expression in amygdala of PTSD model rats reduced obviously (P<0.05). Conclusions:The phosphorylated Akt and mTOR protein expression decreased in amygdala of PTSD rats, which may be concerned with increasing of neurons apoptosis in amygdala during PTSD.