西北药学杂志
西北藥學雜誌
서북약학잡지
2013年
5期
497-500
,共4页
银杏叶提取物(EGb 761)%胰岛素%淀粉样纤维化%细胞毒性
銀杏葉提取物(EGb 761)%胰島素%澱粉樣纖維化%細胞毒性
은행협제취물(EGb 761)%이도소%정분양섬유화%세포독성
Ginkgo biloba leave extract (EGb 761)%insulin%amyloid fibrillation%cytotoxicity
目的探索银杏叶提取物（EGb 761）对胰岛素淀粉样纤维化及纤维细胞毒性的抑制作用。方法在pH 2．0、57℃孵育胰岛素，采用硫黄素荧光检测牛胰岛素形成淀粉样纤维的动力学曲线，透射电镜观察纤维形态；以淀粉样纤维诱导人红细胞的聚集和溶血为指标，评估EGb 761对胰岛素纤维细胞毒性的抑制作用。结果胰岛素在酸性溶液及57℃的条件下孵育可形成淀粉样纤维，EGb 761能够抑制淀粉样纤维的形成，使纤维形态改变，并能够抑制淀粉样纤维诱导的红细胞聚集和溶血。结论 EGb 761能够抑制胰岛素的淀粉样纤维化，并使胰岛素纤维的细胞毒性降低。
目的探索銀杏葉提取物（EGb 761）對胰島素澱粉樣纖維化及纖維細胞毒性的抑製作用。方法在pH 2．0、57℃孵育胰島素，採用硫黃素熒光檢測牛胰島素形成澱粉樣纖維的動力學麯線，透射電鏡觀察纖維形態；以澱粉樣纖維誘導人紅細胞的聚集和溶血為指標，評估EGb 761對胰島素纖維細胞毒性的抑製作用。結果胰島素在痠性溶液及57℃的條件下孵育可形成澱粉樣纖維，EGb 761能夠抑製澱粉樣纖維的形成，使纖維形態改變，併能夠抑製澱粉樣纖維誘導的紅細胞聚集和溶血。結論 EGb 761能夠抑製胰島素的澱粉樣纖維化，併使胰島素纖維的細胞毒性降低。
목적탐색은행협제취물（EGb 761）대이도소정분양섬유화급섬유세포독성적억제작용。방법재pH 2．0、57℃부육이도소，채용류황소형광검측우이도소형성정분양섬유적동역학곡선，투사전경관찰섬유형태；이정분양섬유유도인홍세포적취집화용혈위지표，평고EGb 761대이도소섬유세포독성적억제작용。결과이도소재산성용액급57℃적조건하부육가형성정분양섬유，EGb 761능구억제정분양섬유적형성，사섬유형태개변，병능구억제정분양섬유유도적홍세포취집화용혈。결론 EGb 761능구억제이도소적정분양섬유화，병사이도소섬유적세포독성강저。
Objective To investigate the inhibitory effect of Ginkgo biloba leave extract (EGb 761) on amyloid fibrillation and fibril-lar cytotoxicity of insulin .Methods Insulin fibrils were prepared by incubating the protein at pH 2 .0 and 57 ℃ .Fibril formation was monitored by thioflavine T fluorescence ,and the fibrillar morphology was visualized under transmission electron microscopy . The cytotoxicity of insulin fibril was evaluated by measuring hemolysis and aggregation of human red blood cell treated with the fibrils .Results Incubation of insulin under acidic medium at 57 ℃ resulted in the formation of amyloid fibril .The growth kinetics and morphology of the fibril were changed by co-incubating insulin and EGb 761 .Insulin fibrils induced aggregation and hemolysis of human red blood cell .EGb 761 attenuated the fibril-induced cytotoxicity .Conclusion EGb 761 is able to inhibit the amyloid formation of insulin and to decrease the fibril-induced cytotoxicity .
