CAJ | 학술논문

目的建立 HPLC法测定盆炎净胶囊中芍药苷的含量测定方法。方法采用Kromasil KR100-5 C18色谱柱（250 mm ×4．6 mm ，5μm）；以乙腈-0．5 mL · L -1三乙胺溶液（用磷酸调节pH值至2．5）（14∶86）为流动相；流速为1．0 mL · min-1；检测波长为230 nm。结果芍药苷的线性范围为68～1692 ng ，回归方程为Y＝1．3424 X＋0．2891，r＝1．000；平均回收率为100．3％， RSD为1．8％（n＝9）。结论该方法结果准确，灵敏度高，重复性好，可用于盆炎净胶囊的质量控制。
목적건립 HPLC법측정분염정효낭중작약감적함량측정방법。방법채용Kromasil KR100-5 C18색보주（250 mm ×4．6 mm ，5μm）；이을정-0．5 mL · L -1삼을알용액（용린산조절pH치지2．5）（14∶86）위류동상；류속위1．0 mL · min-1；검측파장위230 nm。결과작약감적선성범위위68～1692 ng ，회귀방정위Y＝1．3424 X＋0．2891，r＝1．000；평균회수솔위100．3％， RSD위1．8％（n＝9）。결론해방법결과준학，령민도고，중복성호，가용우분염정효낭적질량공제。
Objective To establish a method for determination of paeoniflorin in Penyanjing Capsules by HPLC .Methods The Kro-masil KR100-5 C18 (250 mm × 4 .6 mm ,5 μm) was used ,and the mobile phase was consisted of acetonitrile and 0 .5 mL · L -1 triethylamine (adjusting to pH2 .5 with phosphoric acid) (14∶86) at the flow rate of 1 .0 mL · min-1 .The detection wavelength was 230 nm .Results A good linear correlation of paeoniflorin was observed within the range of 68-1 692 ng (r= 1 .000) .The average recovery was 100 .3% with RSD 1 .8% (n=9) .Conclusion The method is accurate ,sensitive and repeatable ,and can be used for the quality control of Penyanjing Capsules .