CAJ | 학술논문

目的建立同时测定金钱草和广金钱草中绿原酸、槲皮素和山柰酚含量的RP-HPLC方法。方法色谱柱为Dikma Dia-monsilTM C18柱（250 mm ×4．6 mm ，5μm）；流动相为甲醇-4 mL · L -1甲酸，梯度洗脱；流速为1．0 mL · min-1；检测波长360 nm。结果绿原酸、槲皮素和山柰酚的线性范围分别为42．08～84．16 ng （ r＝0．9998），19．13～382．68 ng （ r＝0．9995）和16．96～339．20 ng（r＝0．9995），平均加样回收率分别为99．91％，97．92％和99．31％，方法精密度 RSD分别为1．6％，1．4％和1．6％（n＝6）。结论该方法快速、简便、准确度高、重复性好、专属性强，可用于金钱草和广金钱草的质量控制。
목적건립동시측정금전초화엄금전초중록원산、곡피소화산내분함량적RP-HPLC방법。방법색보주위Dikma Dia-monsilTM C18주（250 mm ×4．6 mm ，5μm）；류동상위갑순-4 mL · L -1갑산，제도세탈；류속위1．0 mL · min-1；검측파장360 nm。결과록원산、곡피소화산내분적선성범위분별위42．08～84．16 ng （ r＝0．9998），19．13～382．68 ng （ r＝0．9995）화16．96～339．20 ng（r＝0．9995），평균가양회수솔분별위99．91％，97．92％화99．31％，방법정밀도 RSD분별위1．6％，1．4％화1．6％（n＝6）。결론해방법쾌속、간편、준학도고、중복성호、전속성강，가용우금전초화엄금전초적질량공제。
Objective To simultaneously determine chlorogenic acid ,quercetin and kaempferol in Herba lysimachiae and desmodi-um styracifolium ,an RP-HPLC method was developed .Methods The separation was performed on a C18 (250 mm × 4 .6 mm , 5 μm) column with a gradient elution system of methanol and 4 mL · L -1 formic acid at the flow rate of 1 .0 mL · min-1 .The detection wave length was set at 360 nm .Results The linear ranges of determination for chlorogenic acid ,quercetin and kaempfer-ol were 42 .08-84 .16 ng (r=0 .999 8) ,19 .13-382 .68 ng (r=0 .999 5) and 16 .96-339 .20 ng (r=0 .999 5) ,respectively .The av-erage recoveries were 99 .91% ,97 .92% and 99 .31% ,respectively .The relative standard deviation of precision of the method were 1 .6% ,1 .4% and 1 .6% (n=6) ,respectively .Conclusion The method is rapid ,simple ,and with high accuracy ,good re-peatability ,strong specificity ,and can be used for the Herba lysimachiae and Desmodium styracifolium quality monitoring .