CAJ | 학술논문

目的建立黄芪桂枝五物汤中的黄芪甲苷的含量测定方法。方法采用HPLC-ELSD法测定黄芪桂枝五物汤中黄芪甲苷的含量，色谱条件为：Welchrom-C18色谱柱（250 mm ×4．6 mm ，5μm），以乙腈-水（32∶68）为流动相，流速为1 mL · min-1，柱温25℃；ELSD参数：漂移管温度75℃，喷雾管温度40℃，气体流量1．5 L · min-1，输出压力0．5 M Pa。结果黄芪甲苷在0．03～1．22 mg · mL -1范围内呈良好的线性关系（r＝0．9995），平均回收率为99．8％，方法精密度的RSD为2．1％（n＝6）。结论该方法简单、准确、重复性好，可用于黄芪桂枝五物汤的质量控制。
목적건립황기계지오물탕중적황기갑감적함량측정방법。방법채용HPLC-ELSD법측정황기계지오물탕중황기갑감적함량，색보조건위：Welchrom-C18색보주（250 mm ×4．6 mm ，5μm），이을정-수（32∶68）위류동상，류속위1 mL · min-1，주온25℃；ELSD삼수：표이관온도75℃，분무관온도40℃，기체류량1．5 L · min-1，수출압력0．5 M Pa。결과황기갑감재0．03～1．22 mg · mL -1범위내정량호적선성관계（r＝0．9995），평균회수솔위99．8％，방법정밀도적RSD위2．1％（n＝6）。결론해방법간단、준학、중복성호，가용우황기계지오물탕적질량공제。
Objective To establish an HPLC-ELSD method for determination of astragaloside Ⅳ in Huangqi Guizhi Wuwu Decoc-tion .Methods The astragaloside Ⅳ in the decoction was determined by HPLC-ELSD .The experiment conditions were as fol-lows:Welchrom-C18 (250 mm × 4 .6 mm ,5 μm) column and a mixture of acetonitrile-water (32∶68) as mobile phase .The flow rate was 1 .0 mL · min-1 and the temperature of drift tube for measurement was 75 ℃ .The pressure of air was 0 .5 MPa and the flow rate of carrier gas was 1 .5 L · min-1 .Results There was a good linearity within the range of 0 .03-1 .22 mg · mL -1 (r=0 .999 5) for astragaloside Ⅳ ,and the average recovery was 99 .8% with RSD of 2 .1% .Conclusion The method is simple ,accu-rate and reproducible .It can be used as the quality control method of Huangqi Guizhi Wuwu Decoction .