CAJ | 학술논문

采用乙醇溶剂浸提法提取广元地区油橄榄叶多酚物质。通过单因素和正交试验研究了料液比、乙醇体积分数、浸提温度以及浸提时间对橄榄叶多酚提取量的影响。结果表明：影响橄榄叶多酚提取量的主要因素及顺序为浸提温度＞乙醇体积分数＞浸提时间＞料液比。广元地区油橄榄叶多酚的最佳提取工艺条件为：料液比1∶35（g/mL），乙醇体积分数为60％，浸提温度55℃，浸提时间1.75 h，油橄榄叶多酚提取量为57.22 mg/g，其对DP-PH自由基的半清除质量浓度EC50为36.16μg/mL。
채용을순용제침제법제취엄원지구유감람협다분물질。통과단인소화정교시험연구료료액비、을순체적분수、침제온도이급침제시간대감람협다분제취량적영향。결과표명：영향감람협다분제취량적주요인소급순서위침제온도＞을순체적분수＞침제시간＞료액비。엄원지구유감람협다분적최가제취공예조건위：료액비1∶35（g/mL），을순체적분수위60％，침제온도55℃，침제시간1.75 h，유감람협다분제취량위57.22 mg/g，기대DP-PH자유기적반청제질량농도EC50위36.16μg/mL。
Polyphenols were extracted from olive leaf in the northern region of Sichuan province by ethanol extraction .The extrac-ted factors, such as solid-liquid ratio, concentration of ethanol,extraction temperature and time, were studied.The results of single-factor and orthogonal test showed that the order of the factors which significantly influenced the extraction of polyphenols was extracting temperature, concentration of ethanol , extracting time and solid-liquid ratio.The optimal condition for extraction was that solid-liquid ratio was 1:35, concentration of ethanol was 60%,extracting temperature was 55℃ and extracting time was 1.75h.The extraction yield of polyphenols was 57.22mg/g.The EC50 (Half elimination concentration ) of DPPH radical by olive leaf polyphenols was 36. 16μg/mL.