热带农业科学
熱帶農業科學
열대농업과학
CHINESE JOURNAL OF TROPICAL AGRICULTURE
2013年
5期
33-37
,共5页
橡胶树%次生体胚发生%体胚植株%染色体数目%DNA含量
橡膠樹%次生體胚髮生%體胚植株%染色體數目%DNA含量
상효수%차생체배발생%체배식주%염색체수목%DNA함량
Hevea brasiliensis%secondary embryogenesis%somatic plants%chromosome number%DNA content
遗传稳定是离体繁殖植物种苗的关键。本研究利用染色体制片和流式细胞术，对1～10个增殖世代的次生体胚植株的染色体数目及DNA含量进行了分析，发现1～10个增殖世代的次生体胚植株染色体数目以2n＝36为主，且DNA含量与芽接无性系相同，说明1～10个增殖世代的次生体胚植株在染色体水平保持遗传稳定。
遺傳穩定是離體繁殖植物種苗的關鍵。本研究利用染色體製片和流式細胞術，對1～10箇增殖世代的次生體胚植株的染色體數目及DNA含量進行瞭分析，髮現1～10箇增殖世代的次生體胚植株染色體數目以2n＝36為主，且DNA含量與芽接無性繫相同，說明1～10箇增殖世代的次生體胚植株在染色體水平保持遺傳穩定。
유전은정시리체번식식물충묘적관건。본연구이용염색체제편화류식세포술，대1～10개증식세대적차생체배식주적염색체수목급DNA함량진행료분석，발현1～10개증식세대적차생체배식주염색체수목이2n＝36위주，차DNA함량여아접무성계상동，설명1～10개증식세대적차생체배식주재염색체수평보지유전은정。
s Genetic stability is a key factor for propagation of plants in vitro. Herein, analysis of chromosome number and DNA content of secondary somatic Hevea brasiliensis plants were carried out through chromosome number and flow cytometry. In the results, cells (2n=36), derived from one to ten propagation generations, were mainly observed, and DNA content was same with that of budded plants, indicated that chromosome number and DNA content to not change with increasing of propagation generation.
