郑州大学学报(医学版)
鄭州大學學報(醫學版)
정주대학학보(의학판)
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2013年
5期
637-640
,共4页
饶玉梅%陈刚%李留霞%李秀芳%余静丽
饒玉梅%陳剛%李留霞%李秀芳%餘靜麗
요옥매%진강%리류하%리수방%여정려
miR-106b%宫颈癌%HeLa细胞%顺铂%Twist1%PTEN%p-AKT
miR-106b%宮頸癌%HeLa細胞%順鉑%Twist1%PTEN%p-AKT
miR-106b%궁경암%HeLa세포%순박%Twist1%PTEN%p-AKT
miR-106 b%cervical cancer%HeLa cell%cisplatin%Twist1%PTEN%p-AKT
目的:探讨miR-106 b对宫颈癌HeLa细胞顺铂化疗敏感性的影响。方法:采用实时定量PCR检测转染miR-106b抑制剂和类似物后HeLa细胞中miR-106b的表达,MTT法及流式细胞仪检测转染后HeLa细胞对顺铂的敏感性和凋亡,Western blot检测细胞中Twist1、PTEN、p-AKT(Ser473)蛋白的表达。结果:miR-106b抑制剂可减少HeLa细胞中miR-106b的表达;而miR-106b类似物可增加HeLa细胞中miR-106b的表达。转染miR-106b抑制剂后,细胞对顺铂的敏感性增强、凋亡及PTEN蛋白表达增加,Twist1、p-AKT表达降低(P<0.05);转染miR-106b类似物后,细胞对顺铂的敏感性减弱,凋亡及PTEN蛋白表达降低,Twist1、p-AKT表达增加(P<0.05)。结论:miR-106 b可能通过调节Twist1、PTEN和p-AKT的表达影响HeLa细胞对顺铂耐药。
目的:探討miR-106 b對宮頸癌HeLa細胞順鉑化療敏感性的影響。方法:採用實時定量PCR檢測轉染miR-106b抑製劑和類似物後HeLa細胞中miR-106b的錶達,MTT法及流式細胞儀檢測轉染後HeLa細胞對順鉑的敏感性和凋亡,Western blot檢測細胞中Twist1、PTEN、p-AKT(Ser473)蛋白的錶達。結果:miR-106b抑製劑可減少HeLa細胞中miR-106b的錶達;而miR-106b類似物可增加HeLa細胞中miR-106b的錶達。轉染miR-106b抑製劑後,細胞對順鉑的敏感性增彊、凋亡及PTEN蛋白錶達增加,Twist1、p-AKT錶達降低(P<0.05);轉染miR-106b類似物後,細胞對順鉑的敏感性減弱,凋亡及PTEN蛋白錶達降低,Twist1、p-AKT錶達增加(P<0.05)。結論:miR-106 b可能通過調節Twist1、PTEN和p-AKT的錶達影響HeLa細胞對順鉑耐藥。
목적:탐토miR-106 b대궁경암HeLa세포순박화료민감성적영향。방법:채용실시정량PCR검측전염miR-106b억제제화유사물후HeLa세포중miR-106b적표체,MTT법급류식세포의검측전염후HeLa세포대순박적민감성화조망,Western blot검측세포중Twist1、PTEN、p-AKT(Ser473)단백적표체。결과:miR-106b억제제가감소HeLa세포중miR-106b적표체;이miR-106b유사물가증가HeLa세포중miR-106b적표체。전염miR-106b억제제후,세포대순박적민감성증강、조망급PTEN단백표체증가,Twist1、p-AKT표체강저(P<0.05);전염miR-106b유사물후,세포대순박적민감성감약,조망급PTEN단백표체강저,Twist1、p-AKT표체증가(P<0.05)。결론:miR-106 b가능통과조절Twist1、PTEN화p-AKT적표체영향HeLa세포대순박내약。
Aim:To explore the effects of miR-106 b on chemosensitivity of HeLa cells to cisplatin and its possible mechanism.Methods:miR-106b inhibitors and mimics were transiently transfected into HeLa cells , and the expression of miR-106b was detected by real-time PCR.Then, the drug sensitivity of cells to cisplatin cell apoptosis and the expression levels of Twist1, PTEN and p-AKT were detected by MTT assay , flow cytometry and Western blot .Results:The expres-sion of miR-106 b was significantly decreased after the miR-106 b inhibitors transfection , and increased after the mimics transfection in HeLa cells.Moreover, knockdown of miR-106b expression dramatically increased cell chemosensitivity and apoptosis effects induced by cisplatin and the expression of PTEN , but decreased the levels of Twist 1 and p-AKT in HeLa (P<0.05).On the contrary, overexpression of miR-106b significantly decreased the cell chemosensitivity and apoptosis effects induced by cisplatin and the expression of PTEN , but enhanced the levels of Twist1 and p-AKT(P<0.05).Con-clusion:Cisplatin chemosensitivity of HeLa cells may be modulated by miR-106 b through changing the expressions of Twist1, PTEN and p-AKT.