CAJ | 학술논문

目的：探讨β-连环素（β-catenin）对人羊膜间充质干细胞（ AMSC）增殖的影响。方法：将β-catenin-绿色荧光蛋白（β-catenin-GFP）重组腺病毒扩增后，转染人AMSC，在荧光倒置显微镜下观察转染效率。采用CKK-8法测定AMSC的细胞生长状况。结果：荧光观察证实β-catenin-GFP融合蛋白在AMSC细胞中获得有效表达，β-cate-nin稳定高表达的AMSCs增殖率明显高于正常的AMSCs，分别为50．0％、82．5％、92．6％、169．1％和128．2％。结论：在AMSC细胞中成功表达了β-catenin-GFP融合蛋白，β-catenin高表达能有效地刺激AMSC的增殖。
목적：탐토β-련배소（β-catenin）대인양막간충질간세포（ AMSC）증식적영향。방법：장β-catenin-록색형광단백（β-catenin-GFP）중조선병독확증후，전염인AMSC，재형광도치현미경하관찰전염효솔。채용CKK-8법측정AMSC적세포생장상황。결과：형광관찰증실β-catenin-GFP융합단백재AMSC세포중획득유효표체，β-cate-nin은정고표체적AMSCs증식솔명현고우정상적AMSCs，분별위50．0％、82．5％、92．6％、169．1％화128．2％。결론：재AMSC세포중성공표체료β-catenin-GFP융합단백，β-catenin고표체능유효지자격AMSC적증식。
Aim:To observe the influence of β-catenin on the proliferation of human amniotic membrane derived mes-enchymal stem cells ( AMSC) .Methods:The adenovirus carrying human β-catenin-GFP was amplified and then transfected into human AMSC , followed by observation of the cells by fluorescent microscope .The life span was detected by CKK-8 methods.Results:The expression of β-catenin-GFP fusion protein was confirmed by immunofluorescence studies .Com-pared with the control normal cell , the proliferation rate of AMSCs whith high β-catenin expression was obviously higher , respectively, 50.0%, 82.5%, 92.6%, 169.1%and 128.2%.Conclusion: The fusion protein can be successfully ex-pressed in human AMSC .The high expression of β-catenin can effectively stimulate AMSC proliferation .