郑州大学学报（医学版）
鄭州大學學報（醫學版）
정주대학학보（의학판）
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2013年
5期
595-598
,共4页
张艳艳%张晓艳%付旭东%刘康栋%赵继敏%董子明%张振中
張豔豔%張曉豔%付旭東%劉康棟%趙繼敏%董子明%張振中
장염염%장효염%부욱동%류강동%조계민%동자명%장진중
紫杉醇%单壁碳纳米管%NGR%MCF-7细胞
紫杉醇%單壁碳納米管%NGR%MCF-7細胞
자삼순%단벽탄납미관%NGR%MCF-7세포
paclitaxel%single-walled carbon nanotube%NGR%MCF-7 cell
目的：制备紫杉醇肿瘤靶向给药系统NGR-SWCNTs-Paclitaxel ，观察其对MCF-7乳癌细胞的影响。方法：实验分为对照组、Paclitaxel组、SWCNTs-Paclitaxel组和NGR-SWCNTs-Paclitaxel组。用荧光物质FITC标记单壁碳纳米管（ SWCNTs ），采用细胞吞噬实验观察MCF-7细胞对各制剂的体外摄取情况，用MTT法观察各制剂对MCF-7细胞的增殖抑制作用，采用流式细胞术检测细胞周期和凋亡率。结果：MCF-7细胞能够有效吞噬NGR-SWCNTs-Pacli-taxel复合物，未经NGR-SWCNTs修饰的Paclitaxel 较少进入肿瘤细胞。 Paclitaxel、SWCNTs-Paclitaxel 和NGR-SWC-NTs-Paclitaxel组的细胞增殖抑制率、细胞周期和细胞凋亡率差异均有统计学意义（P＜0．05）；NGR-SWCNTs-Pacli-taxel对细胞的增殖抑制作用最强，诱导的细胞凋亡最有效，G2/M期阻滞效果也最显著（P＜0．05）。结论：成功制备的NGR-SWCNTs-Paclitaxel复合物有良好的肿瘤靶向性和乳癌细胞增殖抑制作用。
目的：製備紫杉醇腫瘤靶嚮給藥繫統NGR-SWCNTs-Paclitaxel ，觀察其對MCF-7乳癌細胞的影響。方法：實驗分為對照組、Paclitaxel組、SWCNTs-Paclitaxel組和NGR-SWCNTs-Paclitaxel組。用熒光物質FITC標記單壁碳納米管（ SWCNTs ），採用細胞吞噬實驗觀察MCF-7細胞對各製劑的體外攝取情況，用MTT法觀察各製劑對MCF-7細胞的增殖抑製作用，採用流式細胞術檢測細胞週期和凋亡率。結果：MCF-7細胞能夠有效吞噬NGR-SWCNTs-Pacli-taxel複閤物，未經NGR-SWCNTs脩飾的Paclitaxel 較少進入腫瘤細胞。 Paclitaxel、SWCNTs-Paclitaxel 和NGR-SWC-NTs-Paclitaxel組的細胞增殖抑製率、細胞週期和細胞凋亡率差異均有統計學意義（P＜0．05）；NGR-SWCNTs-Pacli-taxel對細胞的增殖抑製作用最彊，誘導的細胞凋亡最有效，G2/M期阻滯效果也最顯著（P＜0．05）。結論：成功製備的NGR-SWCNTs-Paclitaxel複閤物有良好的腫瘤靶嚮性和乳癌細胞增殖抑製作用。
목적：제비자삼순종류파향급약계통NGR-SWCNTs-Paclitaxel ，관찰기대MCF-7유암세포적영향。방법：실험분위대조조、Paclitaxel조、SWCNTs-Paclitaxel조화NGR-SWCNTs-Paclitaxel조。용형광물질FITC표기단벽탄납미관（ SWCNTs ），채용세포탄서실험관찰MCF-7세포대각제제적체외섭취정황，용MTT법관찰각제제대MCF-7세포적증식억제작용，채용류식세포술검측세포주기화조망솔。결과：MCF-7세포능구유효탄서NGR-SWCNTs-Pacli-taxel복합물，미경NGR-SWCNTs수식적Paclitaxel 교소진입종류세포。 Paclitaxel、SWCNTs-Paclitaxel 화NGR-SWC-NTs-Paclitaxel조적세포증식억제솔、세포주기화세포조망솔차이균유통계학의의（P＜0．05）；NGR-SWCNTs-Pacli-taxel대세포적증식억제작용최강，유도적세포조망최유효，G2/M기조체효과야최현저（P＜0．05）。결론：성공제비적NGR-SWCNTs-Paclitaxel복합물유량호적종류파향성화유암세포증식억제작용。
Aim:To prepare targeting drug delivery system NGR-SWCNTs-Paclitaxel and observe its effect on MCF-7 cell.Methods:Single-walled carbon nanotubes loading paclitaxel (SWCNTs-Paclitaxel) and NGR-SWCNTs-Paclitaxel were prepared.The MCF-7 cells were treated with paclitaxel ,SWCNTs-Paclitaxel and NGR-SWCNTs-Paclitaxel labelled with FITC,then phagocytosis test was performed in order to evaluate the intake of MCF-7 cell, cell proliferation was tested by MTT assay, and cell cycle and apoptosis were determined by flow cytometry method .The cells without any treatment was the control.Results:NGR-SWCNTs-Paclitaxel was devoured by MCF-7 cell effectively compared with SWCNTs-Paclitaxel and Paclitaxel.The proliferation inhibition ratio and the apoptosis rate had significant difference among these groups ( P <0.05).The proliferation inhibition ratio and the apoptosis rate of the cells treated by NGR-SWCNTs-Paclitaxel were the highest, and the cells were blocked in G2/M(P<0.05).Conclusion: NGR-SWCNTs-Paclitaxel has been successfully constructed , which could target the MCF-7 cells and inhibit the cell proliferation .
