CAJ | 학술논문

目的：研究不同浓度谷氨酸钠刺激对PC12细胞钠离子通道蛋白1．7（Nav1．7）表达的影响。方法：PC12细胞解冻后，胰蛋白酶消化传代培养，对照组为普通培养基，实验组分别用终浓度为5、10、20、40 nmol/L的谷氨酸钠刺激PC12细胞14 h。14 h后收集细胞，通过免疫荧光、RT-PCR、Western-blot等方法检测Nav1．7和SCN9A mR-NA表达量的变化。结果：各组PC12细胞RT-PCR、免疫荧光、Western-blot结果比较，差异均有统计学意义（ F＝11．821、61．871、23．274，P均＜0．001）；且除RT-PCR结果5 nmol/L谷氨酸钠刺激组与对照组相比外，其余各谷氨酸钠刺激组与对照组相比均升高（P均＜0．05）。结论：PC12细胞Nav1．7的表达与疼痛有关。
목적：연구불동농도곡안산납자격대PC12세포납리자통도단백1．7（Nav1．7）표체적영향。방법：PC12세포해동후，이단백매소화전대배양，대조조위보통배양기，실험조분별용종농도위5、10、20、40 nmol/L적곡안산납자격PC12세포14 h。14 h후수집세포，통과면역형광、RT-PCR、Western-blot등방법검측Nav1．7화SCN9A mR-NA표체량적변화。결과：각조PC12세포RT-PCR、면역형광、Western-blot결과비교，차이균유통계학의의（ F＝11．821、61．871、23．274，P균＜0．001）；차제RT-PCR결과5 nmol/L곡안산납자격조여대조조상비외，기여각곡안산납자격조여대조조상비균승고（P균＜0．05）。결론：PC12세포Nav1．7적표체여동통유관。
Aim:To explore the effects of sodium glutamate on sodium channel protein 1.7 subunit ( Nav1.7) expres-sion in PC12 cells.Methods:After respectively treated by 5, 10, 20, 40 nmol/L sodium glutamate to stimulate PC12 cells for 14 hours, immunofluorescence , RT-PCR,and Western-blot were used to assess the change of Nav 1.7 and SCN9A mR-NA.Results:There were significant differences in RT-PCR, immunofluorescence and Western-blot results(F=11.821, 61.871, 23.274, P<0.001).Compared with control group, the results of sodium glutamate groups significantly increased except RT-PCR result of 5 nmol/L sodium glutamate group(P<0.05).Conclusion:The excitatory amino acid could en-hance the expression of Nav1.7 in PC12 cells.