CAJ | 학술논문

目的：探讨PTEN基因在食管鳞癌细胞中的表达水平及突变情况。方法：采用RT-PCR技术检测高分化的Eca109、低分化的EC 9706和EC1食管鳞癌细胞株细胞中PTEN mRNA的表达水平，并克隆PTEN基因全长，与野生型PTEN基因序列比对，分析突变情况。结果：EC1、EC9706、Eca109细胞株中PTEN mRNA的表达水平分别为（0．06±0．02）、（0．24±0．02）、（0．41±0．01），3株细胞中PTEN mRNA 的表达水平差异有统计学意义（F ＝306．330，P＜0．001），Eca109细胞中PTEN mRNA的表达水平高于 EC9706和 EC1细胞（P＜0．05）。3株细胞中PTEN基因均存在不同程度突变，突变主要集中在外显子5和8。结论：食管鳞癌细胞中PTEN表达水平与细胞的分化程度有关，PTEN基因突变与食管鳞癌的发生发展有关。
목적：탐토PTEN기인재식관린암세포중적표체수평급돌변정황。방법：채용RT-PCR기술검측고분화적Eca109、저분화적EC 9706화EC1식관린암세포주세포중PTEN mRNA적표체수평，병극륭PTEN기인전장，여야생형PTEN기인서렬비대，분석돌변정황。결과：EC1、EC9706、Eca109세포주중PTEN mRNA적표체수평분별위（0．06±0．02）、（0．24±0．02）、（0．41±0．01），3주세포중PTEN mRNA 적표체수평차이유통계학의의（F ＝306．330，P＜0．001），Eca109세포중PTEN mRNA적표체수평고우 EC9706화 EC1세포（P＜0．05）。3주세포중PTEN기인균존재불동정도돌변，돌변주요집중재외현자5화8。결론：식관린암세포중PTEN표체수평여세포적분화정도유관，PTEN기인돌변여식관린암적발생발전유관。
Aim:To study the expression level and mutation of PTEN gene in esophageal squamous cell carcinoma (ESCC) cells.Methods:The expression of PTEN mRNA in three ESCC cell lines (EC9706,EC1 of low differentiation and Eca109 of high differentiation ) was detected by RT-PCR.The full length of PTEN gene in the cell lines mentioned above was cloned , and then the sequences of PTEN gene were blasted with the sequence of wide type PTEN gene on GenBank to analyze the mutation sites.Results: The expression levels of PTEN in EC1, EC9706 and Eca109 cells were(0.06 ± 0.02),(0.24 ±0.02) and (0.41 ±0.01), and there were significant difference among the three cell lines (F=306.330, P <0.001).That was higher in Eca109 cells with well differentiation degree than those in EC 9706 and EC1 cells with low differentiation degree(P<0.05).The mutations of PTEN gene in the three ESCC cell lines were found and the mutation sites mainly focused on exon 5 and 8.Conclusion: The expression level of PTEN has correlation with the differentiation degree and there are mutations of PTEN gene in ESCC .