CAJ | 학술논문

目的用不同浓度丹参酮ⅡA磺酸钠（ STS）注射液对火器伤诱导豚鼠急性肺损伤（ ALI）与细胞因子调节作用的研究，探讨STS对ALI保护作用。方法60只豚鼠随机分为6组：正常对照组（ N）、模型组（A）、生理盐水对照组（P）、低剂量STS治疗组（L）、中剂量STS治疗组（M）、高剂量STS治疗组（H），每组10只。通过高温气体给A、P、L、M、H组豚鼠吸入，制作完成豚鼠ALI动物模型；造模30 min后，再把不同剂量STS分别注射L、M、H组豚鼠腹腔内进行药物干预，P组注射生理盐水作为阳性对照组；观察12 h后，记录致豚鼠ALI时的动脉血气分析、血清肿瘤坏死因子-α（TNF-α）、核因子-κB（NF-κB）、白细胞介素-8（IL-8）含量；处死豚鼠取肺组织，测定肺组织湿/干比值（ W/D），在光镜下对肺组织进行病理学检查，并及时记录其病理变化的情况。结果病理学检查：实验第12小时对照组豚鼠肺泡结构清晰，肺泡壁薄，肺泡内未见水肿液；损伤组第12小时无法分辨肺泡结构，肺内大量炎性细胞浸润伴出血、水肿、透明膜形成；丹参酮ⅡA组实验第12小时肺泡结构仍存在，但肺泡间隔增厚，中性粒细胞浸润明显减轻，肺泡腔内见少量渗液。实验第12小时L、M、H组血清TNF-α、IL-8和NF-κB水平明显低于模型组A组（ P＜0.01），丹参酮组L、M、H组豚鼠第12小时动脉血氧分压、血氧饱和度明显高于A组（ P＜0.01）；L、M、H三组两两比较， M与H组豚鼠实验第12小时血清TNF-α、IL-8、NF-κB水平、动脉血氧分压及血氧饱和度未见统计学差异（ P＞0.05），但均较L组有显著差异（ P＜0.01）。结论 STS注射液对火器伤诱导豚鼠ALI具有明显保护作用，其中STS中剂量保护作用最明显，值得在ALI中推广应用。
목적용불동농도단삼동ⅡA광산납（ STS）주사액대화기상유도돈서급성폐손상（ ALI）여세포인자조절작용적연구，탐토STS대ALI보호작용。방법60지돈서수궤분위6조：정상대조조（ N）、모형조（A）、생리염수대조조（P）、저제량STS치료조（L）、중제량STS치료조（M）、고제량STS치료조（H），매조10지。통과고온기체급A、P、L、M、H조돈서흡입，제작완성돈서ALI동물모형；조모30 min후，재파불동제량STS분별주사L、M、H조돈서복강내진행약물간예，P조주사생리염수작위양성대조조；관찰12 h후，기록치돈서ALI시적동맥혈기분석、혈청종류배사인자-α（TNF-α）、핵인자-κB（NF-κB）、백세포개소-8（IL-8）함량；처사돈서취폐조직，측정폐조직습/간비치（ W/D），재광경하대폐조직진행병이학검사，병급시기록기병리변화적정황。결과병이학검사：실험제12소시대조조돈서폐포결구청석，폐포벽박，폐포내미견수종액；손상조제12소시무법분변폐포결구，폐내대량염성세포침윤반출혈、수종、투명막형성；단삼동ⅡA조실험제12소시폐포결구잉존재，단폐포간격증후，중성립세포침윤명현감경，폐포강내견소량삼액。실험제12소시L、M、H조혈청TNF-α、IL-8화NF-κB수평명현저우모형조A조（ P＜0.01），단삼동조L、M、H조돈서제12소시동맥혈양분압、혈양포화도명현고우A조（ P＜0.01）；L、M、H삼조량량비교， M여H조돈서실험제12소시혈청TNF-α、IL-8、NF-κB수평、동맥혈양분압급혈양포화도미견통계학차이（ P＞0.05），단균교L조유현저차이（ P＜0.01）。결론 STS주사액대화기상유도돈서ALI구유명현보호작용，기중STS중제량보호작용최명현，치득재ALI중추엄응용。
Objective To observe the effect of sulfortanshinone sodium injection ( STS) on acute lung injury , we injected different concentrations of STS to guinea pigs with acute lung injury induced by firearm wound . Methods Firstly,60 guinea pigs were randomized to six groups:normal control group(N),acute lung injury model group(A),physiological saline injection in control group (P),and low dose of STS treatment group (L),middle dose of STS treatment group(M),high dose of STS treatment group (H).Then,we treated A,P,L,M,H groups with high-temperature gas to cause acute lung injury .After half an hour,we injected different doses of STS to L,M,H groups through abdominal injection for treatment of 12 hours.The P group treated with normal saline was used as negative control.Futhermore,we detected the arterial blood gas ,TNF-α,IL-8,NF-κB through ELISA and observed pathological changes in the lung tissues by optical microscope .Results The pathology inspection ,the guinea pigs of comparison group structure of pulmonary alveolus was clear ,the pulmonary alveolus wall was thin ,the pulmonary alveolus had not seen fluid after 12 hours;The guinea pigs of injury group was unable to debate the pulmonary alveolus structure ,in the lung the massive phlogocytes infiltrate with part bleeding , the dropsy, the transparent membrane formated;The guinea pigs of the sulfortanshione sodium injection group , the structure of pulmonary alveolus still existed , but the pulmonary alveolus gap accumulated ,the neutral granular cell infiltration obviously reduced ,the pulmonary alveolus cavity saw few exudation.The L,M,H group guinea pig was tested the 12nd hour blood serum TNF-α,IL-8 and NF-κB level was obviously lower than the injury group (P<0.01);and the L,M,H group oxygen partial pressure in arteries blood was obviously higher than the A group (P<0.01).L,M,H three comparison between two groups ,M and H group guinea pig was tested after 12 hours, blood serum TNF-α, IL-8, NF-κB level and oxyhemoglobin saturation had no difference ( P>0.05 ) ,but had obvious difference with L group .Conclusions The data indicated that the middle dose of sulfortanshinone sodium injection is useful to protect guinea pigs with ALI from firearm injury.