中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
6期
2550-2554
,共5页
子宫内膜肿瘤%受体,LHRH%转化生长因子β1%Smad蛋白质类%移植瘤
子宮內膜腫瘤%受體,LHRH%轉化生長因子β1%Smad蛋白質類%移植瘤
자궁내막종류%수체,LHRH%전화생장인자β1%Smad단백질류%이식류
Endometrial neoplasms%Receptors,LHRH%Transforming growth factor beta 1%Smad proteins%Transplantation tumor
目的探讨不同剂量的醋酸丙氨瑞林对人子宫内膜癌裸鼠皮下移植瘤生长及其可能的作用机制。方法建立子宫内膜癌裸鼠移植瘤模型，选32只雌性荷瘤裸鼠随机分为4组，即空白对照组、醋酸丙氨瑞林干预组（20μg/kg、40μg/kg、80μg/kg），不同剂量醋酸丙氨瑞林组均采取肌肉注射方式连续给药，4周后处死裸鼠，将移植瘤完整取出，测量肿瘤体积、绘制肿瘤生长曲线、计算抑瘤率，并应用免疫组织化学法检测瘤组织中TGF-β1、Smad2/3、Smad7蛋白的表达。结果实验对照组、低、中、高剂量醋酸丙氨瑞林组中移植瘤生长曲线斜率依次减小，生长速度依次减慢。低、中、高剂量醋酸丙氨瑞林抑瘤率分别为（13.03±5.4）％、（26.42±7.3）％、（43.27±6.9）％，组间两两比较差异有统计学意义（P＜0.05）。随着醋酸丙氨瑞林浓度的升高，各干预组TGF-β1、Smad2/3蛋白表达水平明显升高，Smad7蛋白表达水平明显下降，且与对照组相比，差异有统计学意义（ P＜0.05）。结论醋酸丙氨瑞林对子宫内膜癌细胞株Hec-1-B裸鼠皮下移植瘤生长有抑制作用，且呈一定的剂量依赖效应，其作用机制可能与调节TGF-β1/Smads信号转导通路有关。
目的探討不同劑量的醋痠丙氨瑞林對人子宮內膜癌裸鼠皮下移植瘤生長及其可能的作用機製。方法建立子宮內膜癌裸鼠移植瘤模型，選32隻雌性荷瘤裸鼠隨機分為4組，即空白對照組、醋痠丙氨瑞林榦預組（20μg/kg、40μg/kg、80μg/kg），不同劑量醋痠丙氨瑞林組均採取肌肉註射方式連續給藥，4週後處死裸鼠，將移植瘤完整取齣，測量腫瘤體積、繪製腫瘤生長麯線、計算抑瘤率，併應用免疫組織化學法檢測瘤組織中TGF-β1、Smad2/3、Smad7蛋白的錶達。結果實驗對照組、低、中、高劑量醋痠丙氨瑞林組中移植瘤生長麯線斜率依次減小，生長速度依次減慢。低、中、高劑量醋痠丙氨瑞林抑瘤率分彆為（13.03±5.4）％、（26.42±7.3）％、（43.27±6.9）％，組間兩兩比較差異有統計學意義（P＜0.05）。隨著醋痠丙氨瑞林濃度的升高，各榦預組TGF-β1、Smad2/3蛋白錶達水平明顯升高，Smad7蛋白錶達水平明顯下降，且與對照組相比，差異有統計學意義（ P＜0.05）。結論醋痠丙氨瑞林對子宮內膜癌細胞株Hec-1-B裸鼠皮下移植瘤生長有抑製作用，且呈一定的劑量依賴效應，其作用機製可能與調節TGF-β1/Smads信號轉導通路有關。
목적탐토불동제량적작산병안서림대인자궁내막암라서피하이식류생장급기가능적작용궤제。방법건립자궁내막암라서이식류모형，선32지자성하류라서수궤분위4조，즉공백대조조、작산병안서림간예조（20μg/kg、40μg/kg、80μg/kg），불동제량작산병안서림조균채취기육주사방식련속급약，4주후처사라서，장이식류완정취출，측량종류체적、회제종류생장곡선、계산억류솔，병응용면역조직화학법검측류조직중TGF-β1、Smad2/3、Smad7단백적표체。결과실험대조조、저、중、고제량작산병안서림조중이식류생장곡선사솔의차감소，생장속도의차감만。저、중、고제량작산병안서림억류솔분별위（13.03±5.4）％、（26.42±7.3）％、（43.27±6.9）％，조간량량비교차이유통계학의의（P＜0.05）。수착작산병안서림농도적승고，각간예조TGF-β1、Smad2/3단백표체수평명현승고，Smad7단백표체수평명현하강，차여대조조상비，차이유통계학의의（ P＜0.05）。결론작산병안서림대자궁내막암세포주Hec-1-B라서피하이식류생장유억제작용，차정일정적제량의뢰효응，기작용궤제가능여조절TGF-β1/Smads신호전도통로유관。
Objective To investigate the effects and its possible molecular mechanisms of different doses of alarelin on human endometrial carcinoma in nude mice of subcutaneous xenograft .Methods Human endometrial carcinoma models were established by subcutaneous transplantation in nude mice , and then 32 of the mice were randomly divided into 4 groups:control group,alarelin groups(20,40,80 μg/kg,respectively).The different-dose Alarelin were injected into the kinds of mice of groups and the blank control group were injected with the isodose isotonic Na chloride in the same way .The systemic condition of the mice were observed for 4 weeks,then the tumor were removed completely .We measured the size of tumor ,drawed the growth curve ,calculated the inhibition rate of the tumor,then,etected the expression of the TGF-β1,Smad2/3,Smad7 with the immunohistochemical technology . Results The slop of tumor growth curve decreased gradually form control group to high dose of alarelin group ,that means growth rate was slowly by and by .Alarelin 20,40,80 μg/kg respectively inhibited tumor growth by (13.03 ± 5.4 )%, ( 26.42 ±7.3 )%, ( 43.27 ±6.9 )%.The difference was significant ( P <0.05 , respectively ) .The immunohistochemical results showed that the expression of TGF-β1,Smad2/3 rised obviously in treated groups with the increasing dose of Alarelin,inversely,the expression of Smad7 reduced obviously,and the difference of the treated groups was significant ( P<0.05 ,respectively ) .Conclusions Alarelin might inhibit directly the growth of the human endometrial carcinoma in nude mice of subcutaneous xenograft and the effect was dose -dependent , and it may be related to adjust TGF-β1/Smads signal transduction pathways .
