中华临床医师杂志(电子版)
中華臨床醫師雜誌(電子版)
중화림상의사잡지(전자판)
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
6期
2528-2534
,共7页
肖孟景%李利根%李晨曦%胡泉%马丽%张海军
肖孟景%李利根%李晨晞%鬍泉%馬麗%張海軍
초맹경%리리근%리신희%호천%마려%장해군
烧伤%自噬%细胞凋亡%西罗莫司
燒傷%自噬%細胞凋亡%西囉莫司
소상%자서%세포조망%서라막사
Burns%Autophagy%Apoptosis%Sirolimus
目的观察雷帕霉素对大鼠深Ⅱ度烧伤创面自噬的表达及对烧伤创面早期加深的作用。方法 Wistar大鼠背部深Ⅱ度烧伤,治疗组腹腔注射雷帕霉素,对照组腹腔注射等量溶媒,观察两组烫伤后6 h、1 d、2 d、3 d创面组织自噬体,自噬标志蛋白LC3、Beclin-1的表达及变化;TUNEL法检测创面凋亡;同时检测烧伤后创面局部多普勒血流(LDF)值,创面组织中IL-8、Na-K-ATP酶、髓过氧化物酶(MPO)活性和丙二醛( MDA)的含量变化,HE染色及Masson染色显示创面组织坏死及加深程度。结果治疗组与对照组相比,电镜下可见自噬体增多,LC3、Beclin-1表达明显增高,创面凋亡率明显低于对照组;治疗组创面LDF值及Na-K-ATP酶活性比对照组明显升高,IL-8、MPO活性和MDA的含量较对照组明显下降,创面深度较对照组减轻。结论雷帕霉素使大鼠深Ⅱ度烧伤创面自噬增强,抑制烧伤创面早期加深。
目的觀察雷帕黴素對大鼠深Ⅱ度燒傷創麵自噬的錶達及對燒傷創麵早期加深的作用。方法 Wistar大鼠揹部深Ⅱ度燒傷,治療組腹腔註射雷帕黴素,對照組腹腔註射等量溶媒,觀察兩組燙傷後6 h、1 d、2 d、3 d創麵組織自噬體,自噬標誌蛋白LC3、Beclin-1的錶達及變化;TUNEL法檢測創麵凋亡;同時檢測燒傷後創麵跼部多普勒血流(LDF)值,創麵組織中IL-8、Na-K-ATP酶、髓過氧化物酶(MPO)活性和丙二醛( MDA)的含量變化,HE染色及Masson染色顯示創麵組織壞死及加深程度。結果治療組與對照組相比,電鏡下可見自噬體增多,LC3、Beclin-1錶達明顯增高,創麵凋亡率明顯低于對照組;治療組創麵LDF值及Na-K-ATP酶活性比對照組明顯升高,IL-8、MPO活性和MDA的含量較對照組明顯下降,創麵深度較對照組減輕。結論雷帕黴素使大鼠深Ⅱ度燒傷創麵自噬增彊,抑製燒傷創麵早期加深。
목적관찰뢰파매소대대서심Ⅱ도소상창면자서적표체급대소상창면조기가심적작용。방법 Wistar대서배부심Ⅱ도소상,치료조복강주사뢰파매소,대조조복강주사등량용매,관찰량조탕상후6 h、1 d、2 d、3 d창면조직자서체,자서표지단백LC3、Beclin-1적표체급변화;TUNEL법검측창면조망;동시검측소상후창면국부다보륵혈류(LDF)치,창면조직중IL-8、Na-K-ATP매、수과양화물매(MPO)활성화병이철( MDA)적함량변화,HE염색급Masson염색현시창면조직배사급가심정도。결과치료조여대조조상비,전경하가견자서체증다,LC3、Beclin-1표체명현증고,창면조망솔명현저우대조조;치료조창면LDF치급Na-K-ATP매활성비대조조명현승고,IL-8、MPO활성화MDA적함량교대조조명현하강,창면심도교대조조감경。결론뢰파매소사대서심Ⅱ도소상창면자서증강,억제소상창면조기가심。
Objective To study the effect of rapamycin on autophagic responses and burn wound progression in a rat model of deep second-degree burn .Methods A model of deep second-degree burn was established on the back of Wistar rats .The treatment group received rapamycin ,and the control group received carrier solvent by intraperitoneal injection .Appearance of autophagic vacuoles and protein expression of the autophagic markers LC3 and Beclin-1 were observed 6 h,1 d,2 d and 3 d after creating the burn .Wound tissue apoptosis was determined by Terminal-Deoxynucleoitidyl Transferase Mediated Nick End Labeling ( TUNEL) assay.In addition,the Laser Doppler Flowmetry(LDF)value,activity of Na/K-ATpase,myeloperoxidase(MPO)activity,IL-8 secretion and the content of malondialdehyde(MDA)were assayed.Staining with HE and Masson dye confirmed presence of ,and time-dependent evolution of burn wound depth .Results Autophagic responses in burn wounds of the treated group were higher than controls as shown by electron microscopy examination of autophagic vacuoles , Western immunoblotting and immunohistochemical staining of LC 3 and Beclin-1 expression .Significantly fewer TUNEL positive cells were found in burn wounds of the treated group as compared with controls .Both the LDF value and Na/K-ATPase activity were markedly higher in the wound tissue group as compared with controls .Presence of IL-8,MDA and MPO activity in the wound treatment group were much lower than in controls .Burn wound progression in the treatment group was successfully ameliorated .Conclusions Rapamycin enhanced autophagic activity and successfully treated burn would deepening in rats of deep-second degree burn .