CAJ | 학술논문

目的检测半乳糖凝集素3（Galectin-3）、细胞角蛋白19（CK19）和甲状腺过氧化物酶（TPO）在甲状腺腺瘤（TA）、结节性甲状腺肿伴乳头状增生（NGWPH）和甲状腺乳头状癌（PTC）中的表达状况，探讨三种抗体联合检测在甲状腺乳头状癌的早期诊断及良恶性病变鉴别诊断中的临床价值。方法采用EliVi-sionTM plus两步免疫组化方法，检测Galectin-3、CK19和TPO在55例PTC、40例NGWPH和40例TA中的表达状况。结果在55例PTC中，Galectin-3、CK19和TPO阳性率分别为92.73％、96.36％、18.18％。 Galectin-3、CK19在PTC组中的阳性表达率显著高于NGWPH和TA组（ P＜0.01）；而TPO在PTC组中的阳性表达率显著低于NGWPH和TA组，差异具有统计学意义（ P＜0.01）；在PTC组中Galectin-3、CK19及TPO联合检测的阳性率高于NGWPH和TA组，三种抗体同时表达在PTC与甲状腺良性疾病之间的差异具有统计学意义（ P＜0.01）。结论 Galectin-3、CK19和TPO联合检测更有助于PTC的准确诊断，为临床治疗提供可靠的依据。
목적검측반유당응집소3（Galectin-3）、세포각단백19（CK19）화갑상선과양화물매（TPO）재갑상선선류（TA）、결절성갑상선종반유두상증생（NGWPH）화갑상선유두상암（PTC）중적표체상황，탐토삼충항체연합검측재갑상선유두상암적조기진단급량악성병변감별진단중적림상개치。방법채용EliVi-sionTM plus량보면역조화방법，검측Galectin-3、CK19화TPO재55례PTC、40례NGWPH화40례TA중적표체상황。결과재55례PTC중，Galectin-3、CK19화TPO양성솔분별위92.73％、96.36％、18.18％。 Galectin-3、CK19재PTC조중적양성표체솔현저고우NGWPH화TA조（ P＜0.01）；이TPO재PTC조중적양성표체솔현저저우NGWPH화TA조，차이구유통계학의의（ P＜0.01）；재PTC조중Galectin-3、CK19급TPO연합검측적양성솔고우NGWPH화TA조，삼충항체동시표체재PTC여갑상선량성질병지간적차이구유통계학의의（ P＜0.01）。결론 Galectin-3、CK19화TPO연합검측경유조우PTC적준학진단，위림상치료제공가고적의거。
Objective To detect the expression of Galectin-3, cytokeratin 19 and thyroid peroxidase in thyroid adenoma ( TA) ,nodular goiter with papillary hyperplasia ( NGWPH) and papillary thyroid carcinoma ( PTC) ,to explore the clinical value of three antibodies combined detection in the early diagnosis of papillary thyroid carcinoma and differential diagnosis in benign and malignant lesions .Methods EliVisionTM plus two-step method was performed to detected the expression of Galectin-3,cytokeratin 19 and thyroid peroxidase in 55 patients with PTC,40 patients with NGWPH and 40 patients with TA.Results The positive expression rates of Galectin-3,cytokeratin 19 and thyroid peroxidase in PTC were 92.73%,96.36%,18.18%,the positive expression rate in PTC significantly higher than NGWPH and TA group ( P<0.01 );The positive expression rates of thyroid peroxidase in PTC significantly lower than NGWPH and TA group ( P <0.01 );The positive expression rates of combined detection of Galectin-3, cytokeratin 19 and thyroid peroxidase in PTC significantly higher than NGWPH and TA group , simultaneously expression of three antibodies between PTC and benign thyroid diseases had a significant difference ( P <0.01 ) . Conclusions Combined detection of Galectin-3,cytokeratin 19 and thyroid peroxidase contribute to more accurate diagnosis of PTC and provide a reliable basis for clinical treatment .