CAJ | 학술논문

研究了一株源自江西德兴铜矿矿区的中温嗜酸兼性异养菌Acidiphilium sp. DX1-1的分离、鉴定、特征及其浸矿行为。菌株Acidiphilium sp. DX1-1为短杆状革兰氏阴性菌，最适合的生长温度为30°C，最适合的生长pH约为3.5。该菌株具有广泛的底物利用特性，可以利用有机物进行异养生长并在细胞内积累聚羟基丁酸酯，也可以利用单质硫、三价铁等无机物进行自养生长。系统发育分析表明 DX1-1属于 Acidiphilium属，与Acidiphilium cryptum and Acidiphilium multivorum的同源性大于99%。在铁闪锌矿生物浸出过程中，Acidiphilium sp. DX1-1表现出极强的浸矿能力，其作用不仅仅是之前报道的作为其他自养嗜酸浸矿细菌的辅助者。在初始pH3.5时，DX1-1能够在一个月内单独地浸出铁闪锌矿中40%的锌。该浸出率高于它与A. ferrooxidans混合以及A. ferrooxidans 单独浸出铁闪锌矿(初始pH均为2.0)的浸出率。
연구료일주원자강서덕흥동광광구적중온기산겸성이양균Acidiphilium sp. DX1-1적분리、감정、특정급기침광행위。균주Acidiphilium sp. DX1-1위단간상혁란씨음성균，최괄합적생장온도위30°C，최괄합적생장pH약위3.5。해균주구유엄범적저물이용특성，가이이용유궤물진행이양생장병재세포내적루취간기정산지，야가이이용단질류、삼개철등무궤물진행자양생장。계통발육분석표명 DX1-1속우 Acidiphilium속，여Acidiphilium cryptum and Acidiphilium multivorum적동원성대우99%。재철섬자광생물침출과정중，Acidiphilium sp. DX1-1표현출겁강적침광능력，기작용불부부시지전보도적작위기타자양기산침광세균적보조자。재초시pH3.5시，DX1-1능구재일개월내단독지침출철섬자광중40%적자。해침출솔고우타여A. ferrooxidans혼합이급A. ferrooxidans 단독침출철섬자광(초시pH균위2.0)적침출솔。
The isolation and characterization of a subspecies of Acidiphilium that not only acts as an enhancer of other autotrophic acidophiles in bioleaching, but also has significant leaching capacity towards marmatite was described. Acidiphilium sp. DX1-1, a Gram-negative, motile, short rod-shaped bacterium that accumulates intracellular polyhydroxybutyrate, was isolated from the Dexing copper mine area in China. It is mesophilic and acidophilic with an optimum growth at 30 °C and pH 3.5. Phylogenetic analyses identify that it is a member of genus Acidiphilium and closely related to Acidiphilium cryptum and Acidiphilium multivorum. It is mixotrophic, utilizing organic substrates and a range of inorganic substrates, such as sulfur, ferric iron and a variety of sulfide minerals. Acidiphilium sp. DX1-1 is able to bioleach 40%of the zinc content of marmatite with the initial pH 3.5 within a month, which is even higher than that of A. ferrooxidans or the mixed culture with A. ferrooxidans at even lower pH.