CAJ | 학술논문

目的构建尿路致病性大肠杆菌CFT073的聚磷酸盐激酶1（ polyphosphate kinase ， PPK1）基因敲除株，并初步探索其体外生物学特性。方法以CFT073株为研究对象，利用Red同源重组技术敲除CFT073的ppk1基因，构建敲除株△pk1；通过细胞实验即以人膀胱癌上皮细胞5637为体外模型对CFT073野生型菌株、敲除菌株的黏附及侵袭能力进行比较；采用96孔板结晶紫染色法分析CFT073 ppk1基因敲除对其生物膜形成能力的影响。结果成功构建了CFT073 ppk1基因敲除株；敲除ppk1后，细菌对膀胱癌上皮细胞的黏附、侵袭能力较野生株都明显减弱；△pk1在各时间点570 nm处结晶紫吸光度值均较野生株的低。结论利用Red同源重组技术可成功敲除CFT073的ppk1基因，ppk1在尿路致病性大肠杆菌黏附、侵袭尿路上皮及生物膜形成过程中可能发挥了重要作用。
목적구건뇨로치병성대장간균CFT073적취린산염격매1（ polyphosphate kinase ， PPK1）기인고제주，병초보탐색기체외생물학특성。방법이CFT073주위연구대상，이용Red동원중조기술고제CFT073적ppk1기인，구건고제주△pk1；통과세포실험즉이인방광암상피세포5637위체외모형대CFT073야생형균주、고제균주적점부급침습능력진행비교；채용96공판결정자염색법분석CFT073 ppk1기인고제대기생물막형성능력적영향。결과성공구건료CFT073 ppk1기인고제주；고제ppk1후，세균대방광암상피세포적점부、침습능력교야생주도명현감약；△pk1재각시간점570 nm처결정자흡광도치균교야생주적저。결론이용Red동원중조기술가성공고제CFT073적ppk1기인，ppk1재뇨로치병성대장간균점부、침습뇨로상피급생물막형성과정중가능발휘료중요작용。
Objective To construct a Polyphosphate kinase 1 ( ppk1) gene deletion mutant of uro-pathogenic Escherichia coli (E.coli) CFT073, and to explore the biological properties of the mutant strain . Methods The ppk1 gene deletion strain (△pk1) was constructed based on CFT073 E.coli strain by usingλRed homologous recombination technology .A comparison analysis was conducted on adhesive and invasive abilities between CFT073 wild type strain and △pk1 strain in in vitro model of human bladder cancer epithe-lial cell 5637 .Crystal violet staining method was used to evaluate the influences of ppk1 gene deletion on biofilm formation.Results The CFT073 ppk1 deletion mutant strain was successfully constructed .Com-pared with the wild type strain ,△pk1 strain showed impaired adhesive and invasive abilities to 5637 cells. Moreover , the absorbance values of crystal violet at 570 nm at each time point of the mutant strain group were also lower than those of the wild-type strain group .Conclusion The ppk1 gene deletion mutant of uro-pathogenic E.coli CFT073 could be successfully constructed by Red homologous recombination technology and its biological properties indicates that ppk1 gene plays an important role in the pathogenesis of uropatho-genic E.coli infection through regulating the abilities of adhesion , invasion and biofilm formation .