CAJ | 학술논문

目的探讨Ad-hIFN-λ1重组腺病毒对胃腺癌细胞SGC-7901增殖的影响及其机制。方法分别用Ad-hIFN-λ1重组腺病毒、Ad-LacZ 空质粒及PBS对照组作用于人胃腺癌细胞SGC-7901，MTT法测定细胞增殖情况，RT-PCR检测胃癌SGC-7901中IFN-λ1 mRNA的表达，免疫荧光法检测hIFN-λ1蛋白的表达，Tunel、流式细胞术检测细胞凋亡。结果 Ad-hIFN-λ1转染胃腺癌细胞SGC-7901后，肿瘤细胞生长明显抑制，细胞中hIFN-λ1 mRNA、蛋白高效表达，细胞凋亡率明显高于Ad-LacZ组、PBS空白对照组。结论 Ad-hIFN-λ1重组腺病毒转染至胃腺癌细胞SGC-7901后表达IFN-λ1，Ad-hIFN-λ1能够显著抑制人胃腺癌SGC-7901细胞生长，并可能通过诱导其凋亡而起作用。
목적탐토Ad-hIFN-λ1중조선병독대위선암세포SGC-7901증식적영향급기궤제。방법분별용Ad-hIFN-λ1중조선병독、Ad-LacZ 공질립급PBS대조조작용우인위선암세포SGC-7901，MTT법측정세포증식정황，RT-PCR검측위암SGC-7901중IFN-λ1 mRNA적표체，면역형광법검측hIFN-λ1단백적표체，Tunel、류식세포술검측세포조망。결과 Ad-hIFN-λ1전염위선암세포SGC-7901후，종류세포생장명현억제，세포중hIFN-λ1 mRNA、단백고효표체，세포조망솔명현고우Ad-LacZ조、PBS공백대조조。결론 Ad-hIFN-λ1중조선병독전염지위선암세포SGC-7901후표체IFN-λ1，Ad-hIFN-λ1능구현저억제인위선암SGC-7901세포생장，병가능통과유도기조망이기작용。
Objective To explore the effects of Ad-hIFN-λ1 recombinant adenovirus on prolifera-tion of gastric adenocarcinoma cell line SGC-7901 and its mechanism .Methods Ad-hIFN-λ1 recombinant adenovirus and empty plasmid Ad-LacZ were respeetively transfated to human gastric adenocarcinoma SGC-7901 cells.The proliferation of transfected cells was detected by MTT assay .IFN-λ1 gene expression at mR-NA and protein levels in the cells were measured by RT-PCR analysis and immunofluorescence assay , re-spectively .Tunel assay and flow cytometry were used to analyze the rate of cell apoptosis .Results The proliferation of gastric adenocarcinoma SGC-7901 cells were significantly inhibited with Ad-hIFN-λ1 inter-vention in accordance with highly expressed IFN-λ1 at mRNA and protein levels , respectively .The apoptosis rate of Ad-hIFN-λ1 transfected cells was markedly higher than that of the empty plasmid Ad-LacZ group and PBS blank control group .Conclusion The expression of hIFN-λ1 could be detected after transfection of Ad-hIFN-λ1 recombinant adenovirus into gastric adenocarcinoma SGC-7901 cells.Ad-hIFN-λ1 could signifi-cantly inhibit the proliferation of gastric adenocarcinoma SGC-7901 cells by promoting the apoptosis of cancer cells.