CAJ | 학술논문

目的研究调节性T细胞（ Treg）及妊娠诱导的调节性T细胞（ piTreg）对na?ve T细胞增殖的体外抑制，评价其抑制率的差异。方法对孕12．5 d（E12．5d）异基因交配孕鼠C57/B6（♀）× BALB/c（♂）的piTreg和未孕雌鼠C57/B6鼠的 Treg 进行流式细胞术检测，观察piTreg 和Treg 在CD4＋T细胞中比例及其胞内Foxp3表达变化；采用羧基荧光素二醋酸盐琥珀酰亚胺酯（ CFSE ）标记的na?ve T作为效应细胞和丝裂霉素C灭活的CD4-T细胞作为刺激细胞的单向混合淋巴细胞培养体系，比较piTreg和Treg对效应细胞的体外抑制强度。结果 piTreg水平明显高于Treg（P＜0．001），且这些细胞均表达高水平的Foxp3；piTreg 在体外实验中对na?ve T细胞的抑制效应明显高于Treg （ P＜0．006），并受其细胞数量的影响。结论妊娠诱导的调节性T细胞较来自于非孕鼠的Treg对na?ve T细胞有更明显的抑制作用，这一现象很可能是由妊娠中父系抗原刺激诱导的CD4＋CD25＋Treg活性差异造成。
목적연구조절성T세포（ Treg）급임신유도적조절성T세포（ piTreg）대na?ve T세포증식적체외억제，평개기억제솔적차이。방법대잉12．5 d（E12．5d）이기인교배잉서C57/B6（♀）× BALB/c（♂）적piTreg화미잉자서C57/B6서적 Treg 진행류식세포술검측，관찰piTreg 화Treg 재CD4＋T세포중비례급기포내Foxp3표체변화；채용최기형광소이작산염호박선아알지（ CFSE ）표기적na?ve T작위효응세포화사렬매소C멸활적CD4-T세포작위자격세포적단향혼합림파세포배양체계，비교piTreg화Treg대효응세포적체외억제강도。결과 piTreg수평명현고우Treg（P＜0．001），차저사세포균표체고수평적Foxp3；piTreg 재체외실험중대na?ve T세포적억제효응명현고우Treg （ P＜0．006），병수기세포수량적영향。결론임신유도적조절성T세포교래자우비잉서적Treg대na?ve T세포유경명현적억제작용，저일현상흔가능시유임신중부계항원자격유도적CD4＋CD25＋Treg활성차이조성。
Objective To investigate the in vitro inhibitory effects of regulatory T cells ( Treg ) from unpregnant mice and pregnancy-induced regulatory T cells ( piTreg) on the proliferation of na?ve T cells and their differences .Methods The numbers of piTreg cells from allogeneic pregnant mice ( C57/B6 fe-male×BALB/c male) on day 12.5 (E12.5d) of gestation and Treg cells from unpregnant C57/B6 mice were detected respectively by flow cytometry .The percentages of piTreg cells and Treg cells in CD 4+T cells of age-matched female mice and their intracellular expression of Foxp 3 were analyzed .The in vitro inhibitory effects of piTreg cells and Treg cells on the CFSE-labeled na?ve T cells ( effector cells ) were compared in a one-way mixed lymphocyte culture system using mitomycin C-inactivated CD4-T cells as stimulator cells . Results The level of piTreg cells in splenic mononuclear cells was significantly higher than that of Treg cells (P<0.001) from normal mice.Foxp3 was highly expressed in both piTreg cells and Treg cells , howev-er slightly increased in piTreg cells .Moreover , piTreg cells had a significant stronger in vitro inhibitory effect on na?ve T cells proliferation than that of Tregs cells (P<0.006), which was in a cell-dependent manner. Conclusion The present study suggests that the piTreg cells have a stronger inhibitory effect on na ?ve T cell proliferation as compared with Terg cells from unpregnant mice , The differential activity of CD 4+CD25+Treg might be mediated by the paternal antigens during pregnancy .