CAJ | 학술논문

目的探讨慢性HCV感染者外周血中CD56＋T细胞的频数、表型和体外细胞毒功能特征。方法采用流式细胞术检测33例慢性HCV感染者及21例健康对照者外周血CD56＋T细胞的频数和细胞表面活化性受体NKG2C、CD16、NKp46和抑制性受体CD158a、NKG2A的表达水平；检测体外未刺激及K562细胞刺激作用下CD 56＋T细胞毒效应（ CD107a）和细胞因子分泌水平（ IFN-γ和TNF-α），并分析上述3种CD56＋T细胞功能指标之间的关联性。结果与健康对照相比，慢性HCV感染者外周血中CD56＋T细胞在淋巴细胞中的比例明显降低（ P＝00．18）。 CD56＋T细胞表面的活化性受体 NKG2C（P＝0．015）、CD16（P＝0．036）、NKp46（P＝0．001）均有不同程度降低，而抑制性受体CD158a、NKG2A未发现有统计学意义的差异（P＞0．05）。体外未刺激情况下，慢性HCV感染者CD56＋T细胞分泌细胞因子IFN-γ和TNF -α均显著弱于健康对照组（P ＜0．0001）；在K562细胞刺激作用下，慢性HCV感染者 CD56＋T细胞CD107 a水平及分泌细胞因子IFN-γ和TNF-α均呈显著降低趋势（ P＜0．0001），且3种功能指标表达水平密切关联（r＞0．80， P＜0．0001）。结论慢性HCV感染者CD56＋T 细胞频数降低，细胞毒能力和重要细胞因子分泌能力均明显减弱。该结果提示显著受损的CD56＋T细胞功能可能与HCV慢性持续性感染有关。
목적탐토만성HCV감염자외주혈중CD56＋T세포적빈수、표형화체외세포독공능특정。방법채용류식세포술검측33례만성HCV감염자급21례건강대조자외주혈CD56＋T세포적빈수화세포표면활화성수체NKG2C、CD16、NKp46화억제성수체CD158a、NKG2A적표체수평；검측체외미자격급K562세포자격작용하CD 56＋T세포독효응（ CD107a）화세포인자분비수평（ IFN-γ화TNF-α），병분석상술3충CD56＋T세포공능지표지간적관련성。결과여건강대조상비，만성HCV감염자외주혈중CD56＋T세포재림파세포중적비례명현강저（ P＝00．18）。 CD56＋T세포표면적활화성수체 NKG2C（P＝0．015）、CD16（P＝0．036）、NKp46（P＝0．001）균유불동정도강저，이억제성수체CD158a、NKG2A미발현유통계학의의적차이（P＞0．05）。체외미자격정황하，만성HCV감염자CD56＋T세포분비세포인자IFN-γ화TNF -α균현저약우건강대조조（P ＜0．0001）；재K562세포자격작용하，만성HCV감염자 CD56＋T세포CD107 a수평급분비세포인자IFN-γ화TNF-α균정현저강저추세（ P＜0．0001），차3충공능지표표체수평밀절관련（r＞0．80， P＜0．0001）。결론만성HCV감염자CD56＋T 세포빈수강저，세포독능력화중요세포인자분비능력균명현감약。해결과제시현저수손적CD56＋T세포공능가능여HCV만성지속성감염유관。
Objective To explore the cell frequency , phenotypes and in vitro cytotoxic effects of circulating CD56+T cells in the patients with chronic HCV infection .Methods Peripheral blood mononu-clear cells (PBMCs) were isolated from 33 patients with HCV chronic infection and 21 healthy subjects. Multi-color flow cytometry was used to analyze cell frequency , expressions of activating receptors ( NKG2C, CD16 and NKp46) and inhibitory receptors (NKG2A and CD158a) on CD56+T cells.The functional mark-er for cytotoxic effects (CD107a) on circulating CD56+T cells and their cytokines expression (IFN-γand TNF-α) with or without stimulation of K 562 human Leukemia cell line were also analyzed .Then the correla-tions among the expressing levels of CD 107 a, IFN-γand TNF-αwere investigated .Results The frequency of CD56+T cells in periphery lymphocytes were significantly decreased in the patients with chronic HCV in -fection as compared with that in healthy controls ( P=0.018 ).The expressions of activating receptors (NKG2C, CD16 and NKp46) on CD56+T cells from HCV infected patients were decreased (P=0.015 for NKG2C, P=0.036 for CD16 and P=0.001 for NKp46), while there was no significant change in the ex-pressions of inhibitory receptors (P>0.05 for both CD158a and NKG2A).The concentrations of IFN-γand TNF-αsecreted by CD56+T cells in the patients with chronic HCV infection were significantly decreased with or without K562 stimulation (P<0.0001).However, in the presence of K562 cells CD107a expression on CD56+T cells were sharply decreased in the patients (P<0.0001).In absence of K562 cells, there was no significant change in CD107a expression on CD56+T cells from patients and healthy controls (P>0.05). The expressions of CD107a, IFN-γand TNF-αwere closely related under the stimulation of K562(r>0.80, P<0.0001).Conclusion The frequency of CD56+T cells was reduced in patients with chronic HCV infec-tion.Moreover, cytotoxic effects and cytokines production mediated by CD 56+T cells were also significantly impaired, indicating that the dysfunction of circulating CD 56+T cells might be associated with the persist-ence of chronic HCV infection .