CAJ | 학술논문

目的应用RNA干扰（ RNAi）技术，明确干扰素调节因子6（ interferon regulatory factor 6， IRF6）基因表达下调对小鼠腭突融合机制的影响。方法取妊娠（gestation day）13.5 d的小鼠胚胎腭突20对，随机平均分为两组，对照组：正常培养的腭突；实验组：IRF6沉默的腭突。两组腭突培养48 h后扫描电镜检测IRF6基因下调后胚胎腭中缝处以及腭突表面的表面形态变化；TUNEL技术检测腭突中嵴上皮（ MEE ）细胞凋亡。结果在对照组，腭中嵴细胞在腭突融合后发生凋亡，腭突间充质融合；实验组IRF6基因表达下调，腭中嵴细胞也出现部分凋亡，但两侧间充质细胞未完全融合。结论 IRF6基因下调抑制了腭突中嵴上皮细胞的凋亡，这可能是IRF6基因突变与先天性唇腭裂发生相关的病理学基础。
목적응용RNA간우（ RNAi）기술，명학간우소조절인자6（ interferon regulatory factor 6， IRF6）기인표체하조대소서악돌융합궤제적영향。방법취임신（gestation day）13.5 d적소서배태악돌20대，수궤평균분위량조，대조조：정상배양적악돌；실험조：IRF6침묵적악돌。량조악돌배양48 h후소묘전경검측IRF6기인하조후배태악중봉처이급악돌표면적표면형태변화；TUNEL기술검측악돌중척상피（ MEE ）세포조망。결과재대조조，악중척세포재악돌융합후발생조망，악돌간충질융합；실험조IRF6기인표체하조，악중척세포야출현부분조망，단량측간충질세포미완전융합。결론 IRF6기인하조억제료악돌중척상피세포적조망，저가능시IRF6기인돌변여선천성진악렬발생상관적병이학기출。
Objective The influence of the down regulation of interferon regulatory factor 6 ( IRF6 ) gene expression on palatal fusion was investigated to use RNA inference technology .Methods Twenty pairs of GD ( Gestation Day ) 13.5 embryo palatal shelves were harvested and randomly divided equally into two groups .The control group included normal palatal shelves;the experimental group included palatal shelves with IRF 6 gene silencing.The scanning electron microscope (SEM) and TUNEL technology were respectably used to detect surface morphologic change of medial edge epithelium and the MEE cell apoptosis after culturing palatal shelves for 48 h. Results The palatal shelves fused and MEE cells apoptosis occurred on control group ;on the experimental group , IRF6 gene expression was down-regulated and MEE cells partly apoptosis occurred ,but palatal mesenchyme cells did not fuse completely.Conclusions The down regulation of IRF6 gene expression may inhibit the MEE cells apoptosis occurrence .It may be the pathologic basis for the correlation between IRF 6 gene mutation and NCLP .