中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
7期
3019-3023
,共5页
陈哲%安慧玲%陈枚洁%方向阳%张志勇%沈雁英%刘淼冰%张麟%缪国斌
陳哲%安慧玲%陳枚潔%方嚮暘%張誌勇%瀋雁英%劉淼冰%張麟%繆國斌
진철%안혜령%진매길%방향양%장지용%침안영%류묘빙%장린%무국빈
心力衰竭%受体,肾上腺素能β3%老年人%大鼠
心力衰竭%受體,腎上腺素能β3%老年人%大鼠
심력쇠갈%수체,신상선소능β3%노년인%대서
Heart failure%Receptors,adrenergic,beta-3%Aged%Rats
目的本研究探讨随年龄的增长，心力衰竭（简称心衰）大鼠β肾上腺素能受体β3的变化及其与左心室功能的相关性。方法应用主动脉弓缩窄术制备大鼠心衰模型。雄性Wistar大鼠按年龄分为青年组和老年组，每组随机分为假手术组、心衰组。分别于术后0、9周测定四组大鼠左心室舒张末压（LVESP）、左心室收缩末压（LVEDP）、左心室压力最大上升速率（dp/dt max）和左心室压力最大下降速度（dp/dt min）；应用逆转录聚合酶链反应（RT-PCR）方法测定心肌组织β3AR mRNA的表达。结果9周后， LVEDP：老年心衰组＞青年心衰组＞假手术组（均为P＜0.01）；相反，四组比较LVESP、dp/dt max和dp/dt min，老年心衰组＜青年心衰组＜假手术组（均为P＜0.01）。β3 AR mRNA表达随年龄及心衰程度加重而增高，老年心衰组（1.32±0.04）＞青年心衰组（1.15±0.04）＞老年假手术组（0.92±0.12）＞青年假手术组（0.78±0.11）（均为P＜0.01）。结论心衰大鼠随年龄增长心肌β3AR表达明显增高；心衰加重，β3AR的表达与年龄及左心室功能高度相关。
目的本研究探討隨年齡的增長，心力衰竭（簡稱心衰）大鼠β腎上腺素能受體β3的變化及其與左心室功能的相關性。方法應用主動脈弓縮窄術製備大鼠心衰模型。雄性Wistar大鼠按年齡分為青年組和老年組，每組隨機分為假手術組、心衰組。分彆于術後0、9週測定四組大鼠左心室舒張末壓（LVESP）、左心室收縮末壓（LVEDP）、左心室壓力最大上升速率（dp/dt max）和左心室壓力最大下降速度（dp/dt min）；應用逆轉錄聚閤酶鏈反應（RT-PCR）方法測定心肌組織β3AR mRNA的錶達。結果9週後， LVEDP：老年心衰組＞青年心衰組＞假手術組（均為P＜0.01）；相反，四組比較LVESP、dp/dt max和dp/dt min，老年心衰組＜青年心衰組＜假手術組（均為P＜0.01）。β3 AR mRNA錶達隨年齡及心衰程度加重而增高，老年心衰組（1.32±0.04）＞青年心衰組（1.15±0.04）＞老年假手術組（0.92±0.12）＞青年假手術組（0.78±0.11）（均為P＜0.01）。結論心衰大鼠隨年齡增長心肌β3AR錶達明顯增高；心衰加重，β3AR的錶達與年齡及左心室功能高度相關。
목적본연구탐토수년령적증장，심력쇠갈（간칭심쇠）대서β신상선소능수체β3적변화급기여좌심실공능적상관성。방법응용주동맥궁축착술제비대서심쇠모형。웅성Wistar대서안년령분위청년조화노년조，매조수궤분위가수술조、심쇠조。분별우술후0、9주측정사조대서좌심실서장말압（LVESP）、좌심실수축말압（LVEDP）、좌심실압력최대상승속솔（dp/dt max）화좌심실압력최대하강속도（dp/dt min）；응용역전록취합매련반응（RT-PCR）방법측정심기조직β3AR mRNA적표체。결과9주후， LVEDP：노년심쇠조＞청년심쇠조＞가수술조（균위P＜0.01）；상반，사조비교LVESP、dp/dt max화dp/dt min，노년심쇠조＜청년심쇠조＜가수술조（균위P＜0.01）。β3 AR mRNA표체수년령급심쇠정도가중이증고，노년심쇠조（1.32±0.04）＞청년심쇠조（1.15±0.04）＞노년가수술조（0.92±0.12）＞청년가수술조（0.78±0.11）（균위P＜0.01）。결론심쇠대서수년령증장심기β3AR표체명현증고；심쇠가중，β3AR적표체여년령급좌심실공능고도상관。
Objective To investigate the age-related expression of β3-adrenergic receptor (β3-AR) in rats with experimental heart failure .Methods The model of heart failure was established by banding the aortic diameter . Male Wistar rats were divided into young and aging groups according to age ,and each group included sham-operation and heart-failure subgroups.At 0 and 9 weeks post-operation,all below were measured:left ventricular end-diastolic pressure (LVEDP),left ventricular end systolic pressure (LVESP),maximal rate of left ventricular pressure rise (dp/dtmax),and fall (dp/dtmin);myocardial β3-AR mRNA by RT-PCR.Results Left ventricular end-diastolic pressure (LVEDP) were significantly higher in the aged heart-failure group than in young heart-failure group (15.84 ±4.03 vs.10.92 ±1.68 , P<0.01 ) and than the sham-operation groups .While the left ventricular end systolic pressure ( LVESP) and the maximal rate of rise or fall of left ventricular pressure were all significantly lower in the aged heart-failure group than in the young heart-failure group and in the sham-operation groups ( all P<0.01 ) . Myocardial β3-AR mRNA were significantly increased in the heart failure group compared to sham-operation group and was also significantly higher in the aged heart-failure group than in the young heart-failure group ( 0.78 ±0.05 vs.0.65 ±0.09 ,P <0.05 ) .Conclusions These results suggest that there is an age-dependent upregulation of myocardial β3-AR mRNA in both sham-operation rats and in rats with experimental heart failure .β3-AR expression increased significantly when heart failure worsened in aging rats .
