CAJ | 학술논문

目的探讨脂联素在HepG2细胞中改善葡萄糖摄取的机制。方法检测不同浓度脂联素干预对HepG2细胞中脂联素受体2（AdipoR2）表达的影响，进而应用siRNA沉默HepG2细胞AdipoR2，观察脂联素通过上调AdipoR2表达对HepG2细胞糖摄取的影响。结果脂联素浓度为50μg/ml时，干预HepG2细胞后使AMPK和GLUT2 mRNA上调，而使PEPCK和G6Pase mRNA下调（P＜0.05），siRNA沉默AdipoR2后， PEPCK和G6Pase mRNA显著上调，AMPK和GLUT2 mRNA显著下调（P＜0.05）。结论脂联素可以通过增加HepG2细胞AdipoR2的表达，上调细胞内的AMPK表达，增加GLUT2数量，降低PEPCK和G6Pase表达水平，改善细胞葡萄糖摄取。
목적탐토지련소재HepG2세포중개선포도당섭취적궤제。방법검측불동농도지련소간예대HepG2세포중지련소수체2（AdipoR2）표체적영향，진이응용siRNA침묵HepG2세포AdipoR2，관찰지련소통과상조AdipoR2표체대HepG2세포당섭취적영향。결과지련소농도위50μg/ml시，간예HepG2세포후사AMPK화GLUT2 mRNA상조，이사PEPCK화G6Pase mRNA하조（P＜0.05），siRNA침묵AdipoR2후， PEPCK화G6Pase mRNA현저상조，AMPK화GLUT2 mRNA현저하조（P＜0.05）。결론지련소가이통과증가HepG2세포AdipoR2적표체，상조세포내적AMPK표체，증가GLUT2수량，강저PEPCK화G6Pase표체수평，개선세포포도당섭취。
Objective To explore the mechanisms of adiponectin stimulating glucose uptake in HepG 2 cells.Methods HepG2 cells were treated with different concentrations of adiponectin or with siRNA targeting adipnectin receptor ( AdipoR2) in HepG2 cells and the evaluation of glucose uptake via upregulation of AdipoR 2 was determined.Results AMPKα2 and GLUT2 were increased(P<0.05),while PEPCK and G6Pase were decreased in transcriptionallevel(P <0.05)after addition of adiponectin(50 μg/ml)in HepG2.After using siRNA targeting AdipoR2,mRNA expression of PEPCK and G6Pase were increased ( P <0.05 ), while AMPK and GLUT2 were decreased ( P<0.05 ) .Conclusions Adiponectin improves glucose uptake in HepG 2 cells via up regulation of AdipoR2 expression,which increases GLUT2 and AMPK expression and reduce PEPCK and G 6Pase.