CAJ | 학술논문

目的探究白藜芦醇对经典抗氧化信号通路Keap1-Nrf2-ARE中关键蛋白Keap1的调控作用与机制。方法培养MDA-MB-231细胞利用不同浓度的白藜芦醇（5μmol/L和20μmol/L）处理24和48 h， Western blot方法检测细胞内Keap1蛋白和其调控转录因子Nrf2的表达变化。结合泛素-蛋白酶体抑制剂MG-132和溶酶体酶抑制剂氯喹的应用，进一步研究白藜芦醇降解Keap1蛋白的可能途径。结果白藜芦醇明显降低MDA-MB-231细胞内Keap1蛋白的表达水平，具有浓度和时间依赖的倾向性；加入MG-132和氯喹并不影响白藜芦醇的作用。结论白藜芦醇能够明显促进Keap1蛋白降解，进而激活Keap1-Nrf2-ARE信号通路，增强细胞抗氧化应激的能力；而导致Keap1蛋白降解的途径有可能不依赖于泛素-蛋白酶体和溶酶体通路，详细机制仍有待进一步研究。
목적탐구백려호순대경전항양화신호통로Keap1-Nrf2-ARE중관건단백Keap1적조공작용여궤제。방법배양MDA-MB-231세포이용불동농도적백려호순（5μmol/L화20μmol/L）처리24화48 h， Western blot방법검측세포내Keap1단백화기조공전록인자Nrf2적표체변화。결합범소-단백매체억제제MG-132화용매체매억제제록규적응용，진일보연구백려호순강해Keap1단백적가능도경。결과백려호순명현강저MDA-MB-231세포내Keap1단백적표체수평，구유농도화시간의뢰적경향성；가입MG-132화록규병불영향백려호순적작용。결론백려호순능구명현촉진Keap1단백강해，진이격활Keap1-Nrf2-ARE신호통로，증강세포항양화응격적능력；이도치Keap1단백강해적도경유가능불의뢰우범소-단백매체화용매체통로，상세궤제잉유대진일보연구。
Objective To explore the effect and mechanism of resveratrol on Keap 1,which is the key in the classic antioxidant signaling pathway Keap 1-Nrf2-ARE.Methods The cultured MDA-MB-231 cells were treated with different concentrations of resveratrol (5μmol/L and 20μmol/L)for 24 or 48 hours.Western blot was performed to detect the intracellular Keap1 and its transcription factor Nrf2.Furthermore,an ubiquitin-proteasome inhibitor MG-132 and lysosomal enzyme inhibitor chloroquine , were applied to figure out the potential ways in which resveratrol could degrade Keap1 protein.Results Resveratrol significantly reduced the expression level of Keap 1 protein in MDA-MB-231 cells.The effect was in a concentration-and time-dependent.However,the effect of resveratrol on Nrf2 content increased .This role was not inhibited by MG-132 or Chloroquine .Conclusions Resveratrol could dramatically promote the degradation of Keap1 protein,thereby activating the Keap1-Nrf2-ARE signaling pathway and enhancing the ability of antioxidative cells .Our results indicated the degradation of Keap 1 protein induced by resveratrol may not be dependent on the ubiquitin-proteasome or lysosome pathway;and the precise mechanism remains to be further studied .