中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2013年
7期
2977-2980
,共4页
心肌炎%胰岛素样生长因子Ⅰ%抗坏血酸%藻蓝蛋白
心肌炎%胰島素樣生長因子Ⅰ%抗壞血痠%藻藍蛋白
심기염%이도소양생장인자Ⅰ%항배혈산%조람단백
Myocarditis%Insulin-like growth factorⅠ%Ascorbic acid%Phycocyanin
目的探讨藻蓝蛋白对病毒性心肌炎小鼠心肌的保护作用。方法75只BALB/c小鼠，雄性，4周龄，随机分成4组：A组空白对照（n＝15）；B组病毒对照（n＝20）；C组维生素C对照（n＝20）；D组藻蓝蛋白干预（ n＝20）。于病毒接种第15天处死小鼠取心脏，光镜检查心肌病理变化，免疫组化、Western blot检测心肌胰岛素样生长因子-1（IGF-1）的表达。结果（1）藻蓝蛋白组和维生素C组较病毒组生存率提高，病理改变减轻，IGF-1表达降低，差异有统计学意义（P＜0.05）；（2）藻蓝蛋白组和维生素C组比较，后者生存率、病理改变及IGF-1表达低于前者，差异无统计学意义（ P＞0.05）。结论藻蓝蛋白可以影响IGF-1表达，减轻心肌病理损伤，对心肌具有保护作用。
目的探討藻藍蛋白對病毒性心肌炎小鼠心肌的保護作用。方法75隻BALB/c小鼠，雄性，4週齡，隨機分成4組：A組空白對照（n＝15）；B組病毒對照（n＝20）；C組維生素C對照（n＝20）；D組藻藍蛋白榦預（ n＝20）。于病毒接種第15天處死小鼠取心髒，光鏡檢查心肌病理變化，免疫組化、Western blot檢測心肌胰島素樣生長因子-1（IGF-1）的錶達。結果（1）藻藍蛋白組和維生素C組較病毒組生存率提高，病理改變減輕，IGF-1錶達降低，差異有統計學意義（P＜0.05）；（2）藻藍蛋白組和維生素C組比較，後者生存率、病理改變及IGF-1錶達低于前者，差異無統計學意義（ P＞0.05）。結論藻藍蛋白可以影響IGF-1錶達，減輕心肌病理損傷，對心肌具有保護作用。
목적탐토조람단백대병독성심기염소서심기적보호작용。방법75지BALB/c소서，웅성，4주령，수궤분성4조：A조공백대조（n＝15）；B조병독대조（n＝20）；C조유생소C대조（n＝20）；D조조람단백간예（ n＝20）。우병독접충제15천처사소서취심장，광경검사심기병리변화，면역조화、Western blot검측심기이도소양생장인자-1（IGF-1）적표체。결과（1）조람단백조화유생소C조교병독조생존솔제고，병리개변감경，IGF-1표체강저，차이유통계학의의（P＜0.05）；（2）조람단백조화유생소C조비교，후자생존솔、병리개변급IGF-1표체저우전자，차이무통계학의의（ P＞0.05）。결론조람단백가이영향IGF-1표체，감경심기병리손상，대심기구유보호작용。
Objective To investigate the protective effects of phycocyanin on myocardium in mice with viral myocarditis.Methods A total of 75 BALB/c mice were assigned into 4 groups:normal control group(group A,n=15),virus control group(group B,n=20),vitamin C intervention group (group C,n=20),phycocyanin intervention group(group D,n=20).Mice were killed on the 15th day after inoculated,the samples of myocardial tissue were obtained.The pathological changes of myocardial tissue were detected by an optical microscope .The expression of IGF-1 in myocardial tissue was detected by immunohistochemistry and western blot .Results Compared with group B,the histopathologic score and the expression of IGF-1 of group C and group D were lower ,of which the survival rate was higher ( P<0.05 ) .There were differences between group C and group D , but not statistically significant ( P>0.05 ) .Conclusions Phycocyanin may influence the expression of ICF-1 and possibly thus providing protective effects on myocardial tissue .
